氢化物发生-原子荧光光谱法测定血清中硒含量研究

Determination of selenium in serum by HG-AFS

采用氢化物发生-原子荧光光谱法测定血清中的硒含量。通过实验确定介质酸浓盐酸溶液的最适宜体积分数为10%袁还原剂硼氢化钾溶液的最适宜质量分数为1.5%遥采用干法消解、湿法消解、微波消解对样品进行前处理，研究不同的消解方法对硒含量测定的影响，实验结果表明:微波消解法优于其他两种方法，具有污染小、时间短、准确度高的优点。该方法的检出限为0.004滋g/L，标准偏差为2.88%。，加标回收率在94.5%-102.5%之间。微波消解-原子荧光光谱法可以快速测定大批量血清样品，缩短工时、提高效率，适用于临床检测。

Hydride -generation atomic fluorescence spectrometry ( HG -AFS )was used to detectselenium in serum. 1 0 % (v/v) HCl was the best solvent for acidic medium, and 1.5%(m^m) KBH4was the best solvent for the reducing agent. Three different pretreatment methods including dryashing, electricity-plate digestion and microwave digestion were adopted for digesting the serum.The results showed that the microwave digestion was superior to others for its low pollution, shortdigestion times and high accuracy. The detection lim it was 0.004 ug/L. The RSD was 2.88%. Therecovery rate of Se was between 94.5% and 102.5%. The Se in serum can be detected bymicrowave digestion-atomic fluorescence spectrometry precisely and quickly. Thus it has significantinfluence on clinical application.