芦荟大黄素对小鼠非酒精性脂肪性肝炎的治疗作用

Effect of aloe emodin on the treatment of non-alcoholic steatohepatitis mice

目的:研究芦荟大黄素治疗非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)的作用。方法:低蛋白高脂肪饲料喂养昆明小鼠8周,构建小鼠NASH模型;然后将模型小鼠随机分为5组:模型组,芦荟大黄素高、中、低剂量组和茴三硫组,分别予生理盐水、高中低剂量的芦荟大黄素以及茴三硫灌胃4周。12周后处死小鼠,检测小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、三酰甘油、超氧化物歧化酶(SOD)、丙二醛、内毒素、肿瘤坏死因子-α(TNF-α)的含量,HE染色观察小鼠肝脏病理组织学结构。结果:不同剂量的芦荟大黄素均能明显降低高脂小鼠血清中ALT、AST、三酰甘油、内毒素、丙二醛和TNF-α的含量(P<0.01),增加SOD活性(P<0.01),且高剂量组效果最为明显,与茴三硫组相当(P>0.05)。病理切片显示芦荟大黄素可以明显改善NASH肝细胞的脂肪变性、炎症浸润等病理损害。结论:中药单体芦荟大黄素对NASH有明显的治疗效果,能改善肝功能、脂质代谢紊乱,减轻肝脏脂肪变性、炎症浸润。

Objective : To investigate the role of traditional Chinese medicine monomer aloe emodin (AE) in treatment of non-alcoholic steatohepatitis ( NASH ) . Methods : Kunming mice were fed with low protein and high fat diet for 8 weeks to establish the NASH mouse model. Then the mice were randomly divided into NASH model group, low-dose AE group, middle-dose AE group, high-dose AE group, anetholtrithionegroup,which of them were given physiological saline, various doses of AE or anetholtri thione groupfor 4 weeks. General conditions of the animals were observed, before the mice were executed at the end ofweek 12. The serum alanine aminotransferase ( ALT) activity, aspartate aminotransferase (AST) activity, serum triglyceride(TG) activity, serum superoxide dismutase( SOD) levels, serum malondialdehyde( MDA)levels, serum endotoxin levels, serum tumor necrosis factor-a ( TNF-a) levels were determined. The livertissue were prepared to investigate hepatic pathologic structure by hematoxylin and eosin ( HE) staining.Results : Compared to the NASH model group, AE at different doses all could significantly reduce serumALT, AST, TG, endotoxin, MDA and TNF-a level(P <0. 01) ,increase serum SOD activity significantly.The effect of AE high dose group was the best and similar with anetholtrithione group ( P > 0. 05 ) . The pathologicalobservation showed that AE could obviously relieve the hepatocyte steatosis, inflammatory infiltrationand other pathological changes. Conclusion : AE exerts the clear effect on the treatment of NASH via improving liver function, lipid metabolism, relieving liver fatty degeneration and inflammatory infiltration.