滤泡调节性T细胞在肺癌移植瘤小鼠体内的变化及意义

Increasement and significance of follicular regulatory T cells in the murine model of Lewis lung carcinoma

目的:研究滤泡调节性T细胞(follicular regulatory T cells,Tfr)在肺癌移植瘤小鼠体内的变化,并探讨肿瘤微环境对Tfr细胞的影响。方法:采用皮下注射Lewis肺癌细胞株的方式建立肺癌移植瘤小鼠模型,通过流式细胞术检测小鼠腹壁引流淋巴结和肿瘤组织中Tfr细胞以及CD4+CXCR5+T细胞的比例,分析Tfr/CD4+CXCR5+T细胞比值的变化。结果:肺癌移植瘤小鼠腹壁引流淋巴结中Tfr细胞的比例为(4.13±0.35)%,较正常对照组小鼠[(1.97±0.26)%]明显增加(t=4.985,P<0.01),并且Tfr/CD4+CXCR5+T细胞比值为0.39±0.03,较正常对照组小鼠(0.21±0.02)明显增加(t=5.148,P<0.01)。在肿瘤发展的第14天,肺癌移植瘤小鼠肿瘤组织中Tfr细胞比例增加尤为显著,与第7天相比差异具有统计学意义(t=5.617,P<0.01)。结论:肺癌移植瘤小鼠体内Tfr细胞比例的增加以及Tfr/CD4+CXCR5+T细胞比值的失衡,预示着Tfr细胞可能参与小鼠肺癌移植瘤的发展进程。

Objective: To investigate the change and potential significance of follicular regulatory T cells ( Tfr) percentage in murine model of Lewis lung carcinoma mice. Methods : The mice were injected with the Lewis cells to establish murine model of lung carcinoma. The percentage of Tfr cells and CD4 + CXCR5 + T cells in abdominal drainage lymph nodes and tumor tissues were determined by flow cytometer. The change at ratio of Tfr/CD4 + CXCR5 + T cell were analyzed. Results: Compared with the normal control mice (1.97 士0? 26) % ,tumor-burdened mice (4. 13 ± 0? 35 ) % had a significantly higher percentage of Tfr cells in abdominal drainage lymph nodes( t - A. 985 , P < 0. 01 ) . Tfr/CD4 + CXCR5 + T cell ratio in tumor-burdened mice(0.39 ± 0.03) was also obviously increased(t=5.148,_P<0.01) compared with the normal control mice(0. 21 ± 0? 02) ? The percentage of Tfr cells in tumor tissues had a significantly higher on fourteenthday,the difference was statistically significant compared with seventh days(^ =5.617 , P <0. 01) . Conclusion;The increased percentage of Tfr cells in Lewis lung carcinoma mice and the imbalance of Tfr/CD4 +CXCR5 + T cell ratio indicated that Tfr cells may be involved in the progression of lung carcinoma.