Isolation and in-situ bioremediation of halophile oil degradation bacteria

嗜盐石油烃降解菌的筛选及原位修复效果研究（英文）

Crude oil exploration has resulted in alarming pollution. It is thereforenecessary to isolate native bacterial strains for contaminated crude oil bioremediation.In this study, three bacteria strains were isolated from the Dagang Oil Field, Tianjing,China, which belonged to the genera Pseudomonas sp. and Bacillus sp., as determinedby 16S rRNA genera sequence similarity analysis. The optimum degradation conditionsof the three bacteria were studied by single factor experiment, and they were 30 °C, 7.2pH, and 3% salinity, 35 °C, 7.5 pH, and 3% salinity, and 37 °C, 7.5 pH, and 5% salinity,respectively. The results showed that the oil degradation rate was the best when theproportion of the different strains was 2:1:2. Moreover, the most effective treatmentconditions were found by orthogonal test to be 10% inoculum, 0.2% oil content, 7.0 pH,3:1 N:P, 3.5% salinity, and 35 °C, the oil degradation rate reached 71.03% under thiscondition.

石油开发导致的污染令人堪忧,因此,在石油污染场地对土著石油烃降解菌进行分离鉴定,并进行石油的微生物降解研究是十分必要的。在天津大港油田分离出三株石油烃降解菌株,利用16sRNA基因序列相似性分析确定三株菌株分别为Pseudomonassp.和Bacillussp.。通过单因素实验确定了各菌株最适宜的降解条件为:#1菌株最适宜的温度30°C、pH7.2、盐度3%;#2菌株最适宜的温度35°C、pH7.5、盐度3%;#3菌株最适宜的降解温度37°C、pH7.5、盐度5%。菌株按照2:1:2的比例投加时原油降解率最高。通过正交试验分析,在接菌量10%、原油浓度0.2%、pH7.0、N:P为3:1、盐度3.5%、温度35°C的条件下,原油降解率最高,可达到71.03%。