雄激素、雌激素受体在尿道下裂患儿阴茎皮肤中的表达

Distribution of androgen receptor and estrogen receptor in penis skin of patients with hypospadias

目的探讨雄激素受体(AR)及雌激素受体(ER)在尿道下裂患儿阴茎不同部位表皮及真皮中的分布特点。方法收集30例尿道下裂患儿阴茎背侧皮肤(背侧组)、异位尿道口腹侧皮肤(腹侧组)及尿道板皮肤组织(尿道板组),应用免疫组织化学检测各组中AR和ER在表皮和真皮成纤维细胞中的表达情况,Image Pro plus 6.0分析AR和ER在不同部位的表皮和真皮中的光密度(OD)值。结果各组AR、ER主要表达于阴茎皮肤基底细胞及棘细胞的细胞核,也表达于真皮成纤维细胞的细胞核。AR在表皮中的表达,尿道板组低于背侧组和腹侧组(P<0.05);各组AR在真皮成纤维细胞中的表达差异无统计学意义。ER在表皮中的表达,背侧组高于腹侧组和尿道板组(P<0.05);ER在真皮成纤维细胞中的表达,背侧组、腹侧组和尿道板组呈依次升高趋势(P<0.05)。结论阴茎尿道板组织中AR表达数量减少在尿道下裂发生中起重要作用,阴茎皮肤真皮成纤维细胞中ER表达的失衡可能参与了尿道下裂的发生。

Objective To characterize the expressions of androgen receptor (AR) and estrogen receptor (ER) in penis skin of patients with congenital hypospadias. Methods Dorsal prepuce, ventral prepuce, and urethral plate were harvested from 30 patients with congenital hypospadias. The expressions of AR and ER in epidermal cells and dermal fibroblasts were assessed respectively by automated immunohistochemistry. Image Pro plus 6.0 was used to analyze the optical density (OD)value of AR and ER in different parts of epidermal cells and dermal fibroblasts. Results AR and ER were located mainly in nucleis of the squamous basal cells and prickle cells, and were also found in nucleis of subcutaneous fibroblast cells. The expression of AR was lower in epidermis of urethral plate than that of dorsal prepuce and ventral prepuce (P<0.05), but no significant difference was detected in dermal fibroblasts. The expression of ER was higher in epidermis of dorsal prepuce than that of urethral plate and ventral prepuce (P<0.05). The dermal expression of ER in fibroblast cells was increased successively in dorsal prepuce, ventral prepuce and urethral plate (P<0.05). Conclusion Lower expression of AR in urethral plate may contribute the development of hypospadias. Disorder of ER in dermal fibroblast cells of prepuce may play an important role in hypospadias.