基于离子色谱技术的血清电解质参考测量程序测定结果的不确定度评定

Uncertainty of serum electrolyte determinations based on ion chromatography as reference measurement procedures

目的建立一种合理评定基于离子色谱技术建立的血清电解质参考测量程序测定结果不确定度的评估方法。方法参照JJF1059-1999《测量不确定度评定与表示》、JJF1135-2005《化学分析测量不确定度评定》、CNAS-GL06《化学分析中不确定度的评估指南》,从样本峰面积、标准溶液浓度修正因子、样本处理修正因子和测量重复性4个方面评定2014年参考实验室外部质量评价计划(RELA)-A样本中钠、钾、镁、钙离子测定结果的不确定度。结果 2014年RELA-A样本中钠、钾、镁、钙的水平分别为3 177.79、228.84、48.95、127.01 mg/L,标准不确定度分别为5.66、1.38、0.38、0.75 mg/L,扩展不确定度(k=2)分别为11.33、2.76、0.75、1.50 mg/L。结论依据国际检验医学溯源联合委员会(JCTLM)推荐的血清电解质参考测量程序建立的测量不确定度评定方法可满足临床实验室对血清电解质参考测量系统的要求。

Objective To establish a method for evaluating the uncertainty of serum electrolyte determinations basedon ion chromatography as reference measurement procedures. Methods The uncertainties of sodium,potassium,magnesium and calcium determinations in the External Quality Assessment Scheme for Reference Laboratories in LaboratoryMedicine(RELA)-A 2014 sample were evaluated from peak areas of samples,concentration correction factors ofstandard solution,correction factor of sample processing and measurement repeatability,according to JJF1059-1999Evaluation and Expression of Uncertainty in Measurement,JJF1135-2005 Evaluation of Uncertainty in Chemical AnalysisMeasurement and CNAS-GL06 Guidance on Evaluating the Uncertainty in Chemical Analysis. Results The levels ofRELA-A 2014 sample were 3 177.79 mg/L for sodium,228.84 mg/L for potassium,48.95 mg/L for magnesium and127.01 mg/L for calcium. Standard uncertainties were 5.66,1.38,0.38 and 0.75 mg/L. The expanded uncertainties(k=2)were 11.33,2.76,0.75 and 1.50 mg/L,respectively. Conclusions The uncertainty evaluation reference measurementprocedure method has been established for serum electrolyte determinations recommended by the Joint Committee forTraceability in Laboratory Medicine(JCTLM),which can meet the requirements of serum electrolyte determinations forclinical laboratories.