打顶期烟草组织基因表达分析及打顶对腋芽基因表达的影响

Analysis of Expressed Genes at the Topping Stage and Effects of Topping to Genes Expression in Axillary Buds in Nicotiana tabacum

打顶是烟叶生产重要的技术环节之一。为了研究烟草(Nicotiana tabacum)打顶期基因表达模式,选取广西大宁地区晒黄烟现蕾期10个打顶或非打顶的组织样本,构建链特异总RNA测序文库,利用Illumina HiSeq 2000完成测序。通过质量控制,获得9千多万条测序数据,分析获得50 706个基因的表达信息。筛选组织特异和打顶差异表达基因,共富集687个组织特异表达基因和444个腋芽打顶差异表达基因,为烟草组织基因表达模式和打顶分子响应机制研究提供数据支撑,也为调控烟草营养和生殖生长提供分子靶标。

Topping is one of the important technical procedures of Nicotiana tabacum production. In order to study the geneexpression patterns at the topping stage in Nicotiana tabacum, we selected the Daning tobacco growing area in Guangxi andcollected ten tissue samples at the topping stage. The samples were used to extract total RNAs and to construct stranded specific totalRNA sequencing library and to sequence using the Illumina HiSeq 2000 sequencer. Through quality control, more than 91 millionclean reads were obtained and a total of 50706 genes were detected to be expressed from ten tissues at the topping stage. Afternormalization, screening for tissue-specific and genes differentially expressed among tested tissues, a total of 687 genes wereidentified to be tissue-specifical and 444 genes showed differential expression before and after topping. Above data and results willnot only provide some support for exploring the molecular responses of topping, but also provide molecular targets for the regulatiionof Nicotiana tabacum vegetative and reproductive growth.