响应面试验优化超声提取黄秋葵花果胶多糖工艺及其体外抗氧化活性

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology and Antioxidant Activities of Pectic Polysaccharide from Okra Flowers

以黄秋葵花为原料,在单因素试验的基础上,采用响应面分析法,优化了超声辅助提取果胶多糖工艺。结果表明,超声辅助提取黄秋葵花果胶多糖的最优工艺为提取温度55℃、提取时间30 min、超声功率85.5 W、料液比1∶40(g/m L),黄秋葵花果胶多糖提取率为12.62%,与预测值相近,说明模型拟合良好,该优化工艺准确可行。同时,提取的黄秋葵花果胶多糖具有较好的体外抗氧化活性,是一种潜在的天然抗氧化活性成分。

Based on one-factor-at-a-time experiments, response surface methodology was employed to optimize the ultrasound-assisted extraction of pectic polysaccharide from okra flowers. The optimal conditions were determined as follows: extraction temperature, 55 ℃; extraction time, 30 min; ultrasonic power, 85.5 W; and raw material-to-water ratio,1:40 (g/mL). Under these conditions, the experimental yield of pectic polysaccharide was 12.62%, which was well matched with the value predicted by the developed model. Furthermore, the pectic polysaccharide from okra flowers exhibited significant antioxidant activities in vitro and could be developed as a potential natural antioxidant ingredient in the food industry.