胃癌患者外周血miR-183水平的临床意义

Level of miR-183 in peripheral blood of the patients with gastric cancer

目的·研究胃癌患者外周血mi R-183水平在胃癌的诊断及预后判断中的价值。方法·实时定量PCR(q RT-PCR)法检测76例胃癌患者及同期26例正常对照者外周血清mi R-183的水平,分析mi R-183水平与胃癌患者临床病理特征及预后的关系。结果·胃癌患者血清中mi R-183水平明显高于正常对照者(4.798±0.267 vs 0.904±0.067,P=0.000)。mi R-183水平与患者年龄、性别、肿瘤分化程度无明显相关性(P>0.05);与肿瘤浸润深度、有无淋巴结转移或远处转移及TNM分期有关(P<0.05)。受试者工作特征曲线分析结果显示,mi R-183在预测胃癌发生时,ROC曲线下面积(AUC)为0.978(95%CI为0.955~1.001),敏感度为93.42%,特异度为92.31%;在预测胃癌转移(淋巴结转移或远处转移)时,其AUC值为0.643(95%CI为0.507~0.780),敏感度为67.31%,特异度为58.33%。结论·mi R-183在血清中的水平上调与胃癌发生、发展有关,胃癌患者血清中的表达上调与胃癌的临床病理特征及患者预后密切相关,mi R-183可能成为胃癌诊断及预后判断的分子标志。

Objective·To evaluate level of miR-183in peripheral blood of the patients with gastric cancer for diagnosis and prognosis.Methods·Quantitative real-time PCR was used to detect the miR-183levels in sera of76cases of gastric cancer patients and26cases of normal controlat the same term.The correlations of miR-183levels with clinicopathological features and clinical prognosis were analyzed.Results·The significantlyhigher levels of miR-183were observed in the sera of gastric cancer patients(4.798±0.267vs0.904±0.067,P=0.000)as compared with those in thecorresponding control group.Up-regulation of miR-183was associated with invasion depth,positive lymph node or distant metastasis,and advancedclinical stage in gastric cancer patients(P<0.05),but not associated with age,sex,and degree of tumor differentiation(P>0.05).ROC curve analysisshowed that the AUC value of miR-183in the prediction of gastric cancer was0.978(95%CI0.955-1.001)with sensitivity of93.42%and specificity of92.31%.In the prediction of metastasis(lymph node metastasis or distant metastasis),the AUC value was0.643(95%CI0.507-0.780)with sensitivity of67.31%and specificity of58.33%.Conclusion·The high level of miR-183in serum is associated with gastric cancer development,its clinicopathologicalfeatures and patient’s clinical prognosis.It may be used as a potential diagnostic bio-marker and a prognostic predictor in the patients with gastric cancer.