不同消毒方法对藻酸盐印模消毒效果及尺寸稳定性影响的研究

Comparative Study on the Effects of Different Disinfection Methods for Impression Disinfection Efficacy and Dimensional Stability

目的:探讨不同消毒方法对印模消毒效果及尺寸稳定性的影响,为临床应用提供依据。方法:制作藻酸盐试件,表面沾染三种不同种类细菌(大肠杆菌、白色念珠菌、枯草芽孢杆菌)。并用不同消毒方法(Hygojet牙模清洗器、戊二醛浸泡10min、戊二醛浸泡30min、流水冲洗30s)对试件消毒。将试件染菌面与琼脂平板轻轻接触1h,取走试件,将琼脂平板放入培养箱中过夜培养。次日观察平板上菌落,以琼脂平板无菌落为完全杀灭,计算杀灭率。并测量消毒后试件的3条直径,计算直径平均值,与消毒前直径进行对比,比较其尺寸稳定性。结果:各组的消毒效果不尽相同。使用Hygojet牙模清洗器,对印模消毒效果理想,杀灭了100%大肠杆菌,对白色念珠菌及枯草芽孢杆菌的杀灭率也均达到95%。其余几种方法均不甚理想。除流水冲洗组外,其他组印模尺寸均出现变化,Hygojet牙模清洗器组印模形变1%左右,而戊二醛浸泡30min组形变在5%左右。结论:Hygojet牙模清洗器消毒效果最好,印模形变在可接受范围内,可以在临床进一步应用。

Objective This study investigated the effects of different disinfection methods for impression disinfection efficacy and dimensional stability, provide the basis for clinical application. Methods Alginate samples were made and the surfaces were contaminated with three different species of bacteria (Escherichia coli, Candida albicans, Bacillus subtilis). The samples were then sterilized by different disinfection methods (Hygojet Dental Cleaner, glutaraldehyde soak for 10 minutes,glutaraldehyde soak for 30 minutes, and running water for 30 seconds). The test pieces stained with agar gently touch the plate for 1 hour, remove the specimen, the agar plate in the incubator overnight culture. The next day to observe the colonies on the plate to agar plate without colony complete kill, calculate the kill rate. And the three diameters of the specimens after sterilization were measured. The average diameter was calculated and compared with the diameter before sterilization, and the dimensional stability was compared. Results The disinfection effect of each group is different. The use of Hygojet Dental cleaning device, the mold is ideal for disinfection, killing 100% of E. coli, Candida albicans and Bacillus subtilis kill rate also reached 95%. The remaining centralized methods are less than ideal. In addition to running water group, other groups have appeared impression expansion, about Hygojet dental wax cleaner set stamp deformation 1% glutaraldehyde solution for 30 minutes at about 5% deformation group. Conclusion Hygojet dental cleaning agent disinfect the best, impression deformation in the acceptable range, can be further applied in clinical practice.