有机垃圾热解炭对紫色土细菌群落结构的影响

Influence of organic fraction of municipal solid waste-based biochar on microbial community structure in a purples oil

以700℃热解制得城市有机垃圾(OFMSW)生物炭,为研究该生物炭对氮素缺乏的紫色土有机质、氮营养元素以及微生物群落结构的影响,设计了为期12个月的土壤盆栽培养试验.试验共设置生物炭添加量分别为0%、1%、3%和5%(w/w)的4个处理.采用高通量测序技术分析土壤的微生物群落结构.试验表明:3%和5%的生物炭添加量显著提高了土壤有机质和全氮含量,同时显著降低了紫色土中细菌的α-多样性;主成分分析显示,0%与1%添加量处理的细菌群落组成相似,3%与5%添加量处理的细菌群落组成相似.Illumina测序从24个土样中鉴定出了42门细菌,其中主要的6个菌门为变形菌门、酸杆菌门、拟杆菌门、放线菌门、绿弯菌门和芽单胞菌门,它们序列数占序列总数的83.7%~94.3%.各菌门对不同的生物炭添加量响应不同.从24个土壤样本中鉴定出642个菌属,相对丰度大于1%的有105个.部分主要菌属对3%和5%的生物炭添加量的响应与0%和1%的不同.

Organic fraction of municipal solid waste(OFMSW)was pyrolyzed at700℃to produce biochar,which wasused to amend an organic and nitrogen deficient purple soil in Chongqing.Twelve month pot incubation experiments wereconducted to investigate effects of the OFMSW-based biochar on the content of organic matter(OM)and total nitrogen(TN)as well as microbial community structure of the purple soil.The experiments were performed with four treatments of0%,1%,3%and5%(w/w)of biochar addition,and the microbial communities of the amended soils were analyzed byhigh throughput sequencing technology.The results showed that the addition of3%and5%of biochar significantlyincreased the content of OM and TN and reduced the alpha diversity of bacteria in purple soils.The principal componentanalysis showed that the bacterial communities in the0%and1%treatments were similar,and the bacterial communitiesin the3%and5%treatments were similar.The Illumina sequencing from24soil samples identified42bacteria phyla,6ofwhich were the main bacterial phyla Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi andGemmatimonadetes,accounting for83.7%~94.3%of the total number of sequencing.The bacteria phyla varied in theirresponse to the addition levels of biochar.In24soil samples,642genera were identified,among which105genera had arelative abundance greater than1%.The responses of some major bacterial genera to3%and5%addition of biochar weredifferent from their responses to0%and1%biochar addition.