摘要
以蓝圆鲹为原料,采用胰蛋白酶、木瓜蛋白酶及碱性蛋白酶对其进行水解,研究酶解产物与钙、亚铁、锌离子的螯合活性。结果表明:蓝圆鲹经胰蛋白酶水解,水解度为23.14%时其产物具有较高螯合铁、锌离子的活性,其螯合率分别高达96.63%和94.28%;蓝圆鲹经木瓜蛋白酶水解,水解度为22.00%时,其产物具有较高螯合钙离子的活性,其螯合率高达96.78%;同时研究表明上述两种酶解物还具有较好的抗氧化活性,其1,1-二苯基-2-三硝基苯肼半抑制浓度值分别为3.74 mg/m L和3.64 mg/m L。此外,氨基酸分析表明蓝圆鲹高螯合矿物离子活性的酶解物中天冬氨酸、谷氨酸、赖氨酸含量较高。
In this research, we studied the chelating activity of Decapterus maruadsi muscle hydrolyzed by three different proteases, trypsin, papain and alcalase, towards Ca2+, Fe2+ and Zn2+. The results showed that the hydrolysates produced by trypsin (DH = 23.14%) had the highest chelating activity towards Fe2+ and Zn2+ with chelating rates of 96.63% and 94.28%,respectively. The hydrolysates by papain (DH = 22.0%) had the highest Ca2+ chelating activity with a chelating rate of 96.78%. It was also shown that both enzymatic hydrolysates had good antioxidant activity. The half maximal inhibitory concentration (IC50) values of the trypsin and papain hydrolysates for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were 3.74 and 3.64 mg/mL, respectively. In addition, the amino acid sequence analysis showed that the hydrolysates with higher contents of Glu, Asp and Lys had higher mineral ion chelating activity.
作者
杨伊然
胡晓
杨贤庆
李来好
陈胜军
吴燕燕
林婉玲
黄卉
马海霞
YANG Yiran;HU Xiao;YANG Xianqing;LI Laihao;CHEN Shengjun;WU Yanyan;LIN Wanling;HUANG Hui;MA Haixia(Key Laboratory of Aquatic Producut Processing, Ministry of Agriculture, National R&D Center for Aquatic Product Processing,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciencces, Guangzhou 510300, China;College of Food Science and Engineering, Dalian Ocean University, Dalian 116023)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第3期88-93,共6页
Food Science
基金
国家海洋公益性项目(201305018)
国家自然科学基金青年科学基金项目(31301454)
农业部财政重大专项(NFZX2013)
广东省海洋渔业科技与产业发展专项(A201401C02
Z2015008)
“十二五”国家科技支撑计划项目(2015BAD17B03-02)
关键词
蓝圆鲹
酶解物
螯合活性
矿物离子
抗氧化
Decapterus maruadsi
hydrolysate
chelating activity
mineral ions
antioxidant
