摘要
以玉米肽(corn peptide,CP)为参照研究玉米肽-锌(CP-Zn)螯合物的结构表征和体内抗氧化活性变化。采用氨基酸分析、紫外扫描、红外光谱研究结构变化;对小鼠灌胃不同剂量的CP、CP-Zn(低、中、高剂量组分别为50、150、250 mg/(kg·d)),测其血清、肝脏中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,分析比较CP、CP-Zn体内抗氧化活性。结果表明:螯合前后氨基酸组成、紫外吸收光谱、红外吸收光谱存在明显变化,Zn2+与—COOH、—NH_2、C=O进行了配位;CP高剂量、CP-Zn高、中剂量能显著降低小鼠血清、肝脏中MDA含量(P<0.05或P<0.01),提高SOD活力(P<0.01)、GSH-Px活力(P<0.05或P<0.01),CP-Zn抗氧化活性优于CP。由此可见,CP-Zn属于螯合物,同时具有增强CP抗氧化活性的作用。
In this work, in comparison to corn peptide (CP), we investigated the change in structural characteristics and antioxidant activity in vivo of corn peptide-Zn (CP-Zn) complex. The structures of CP and CP-Zn were investigated through amino acid analysis, ultraviolet (UV) spectroscopy and infrared spectroscopy. CP and CP-Zn (50, 150, 250 mg/(kg·d)) at different doses were administered intragastrically to mice. Two days later, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity in serum and liver were measured to evaluate antioxidant activity in vivo of CP and CP-Zn. The results showed a significant change in amino acid content, and UV and FT-IR spectra between CP and CP-Zn. Zn2+ was coordinated with the –COOH, –NH2 and C=O groups of corn peptide.Compared with the control group, high-dose CP and high- and middle-dose CP-Zn could reduce MDA level (P < 0.05 or P < 0.01), and improve SOD (P < 0.01) and GSH-Px activity (P < 0.05 or P < 0.01) in serum and liver. The antioxidant activity of CP-Zn was better than that of CP.
作者
张智
刘慧
刘奇
郑喜群
曲巍
杨可心
ZHANG Zhi;LIU Hui;LIU Qi;ZHENG Xiqun;QU Wei;YANG Kexin(College of Forestry, Northeast Forestry University, Harbin 150040, China;Key Constructive Laboratory of Processing Agricultural Products of Heilongjiang Province Normal University, Qiqihar University, Qiqihar 161006, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第3期131-135,共5页
Food Science
基金
黑龙江省应用技术研究与开发计划重大项目(GA13B201)
关键词
玉米肽-锌螯合物
结构
抗氧化
corn peptide-Zn complex
structure
antioxidant activity