北宗黄酒麦曲微生物的分离鉴定

Isolation and Identification of Microorganisms in Wheat Qu of Beizong Rice Wine

利用梯度稀释法和划线纯化法对麦曲中的微生物进行分离纯化,得到7株细菌和5株真菌。通过形态学特征观察和分子生物学方法进行菌种鉴定,细菌分别为地衣芽孢杆菌(Bacillus licheniformis)、枯草芽孢杆菌(Bacillus subtilis)、短小芽孢杆菌(Bacillus pumilus)、萎缩芽孢杆菌(Bacillus atrophaeus)、克劳氏芽孢杆菌(Bacillus clausii)、根际芽孢杆菌(Bacillus rhizosphaerae)、索诺拉沙漠芽孢杆菌(Bacillus sonorensis)。真菌分别为米曲霉(Aspergillus oryzae)、黑曲霉(Aspergillus niger)、根菌属(Talaromyces radicus)、粗糙脉孢菌(Neurospora crassa)、伞状犁头霉(Absidia corymbifera)。

In this research,we used the gradient dilution and streak plate methods to isolate and purify microorganisms fromwheat Qu for Beizong rice wine.A total of seven bacterial strains and five fungal strains were obtained.Through observationof their morphological characteristics and molecular biological characterization,the bacterial strains were identified asBacillus licheniformis,Bacillus subtilis,Bacillus pumilus,Bacillus atrophaeus,Bacillus clausii,Bacillus rhizosphaerae,andBacillus sonorensis,and the fungal strains were identified as Aspergillus oryzae,Aspergillus niger,Talaromyces radicus,Neurospora crassa,and Absidia corymbifera.