摘要
为筛选得到一株产灵菌红素的黏质沙雷氏菌为发酵菌种,采用响应面法优化培养基组分和发酵条件,提高黏质沙雷氏菌生产灵菌红素的效率,结果表明,黏质沙雷氏菌最优培养基组分及发酵条件为玉米粉用量10 g/L、菜籽饼粕用量21.7 g/L、硫酸锌质量浓度0.05 g/L、发酵液初始pH 5.8、接种量5.5%、装液量80 m L/250 m L三角瓶、温度27℃、摇床转速200 r/min,培养24 h后,发酵液中灵菌红素产量达到11.56 g/L。本研究为高温菜籽饼粕原料发酵生产灵菌红素提供理论依据。
A culture medium containing rapeseed meal was designed for prodigiosin production by Serratia marcescens.Optimizations of culture medium composition and fermentation conditions by response surface method were performedfor improved production of prodigiosin.The results indicated that the optimal medium composition was composed of cornpowder10g/L,rapeseed meal21.7g/L,inoculum concentration5.5%,and zinc sulfate0.05g/L at an initial pH of5.8.When the strain was cultured for24h in80mL of the medium contained in a250mL flask with shaking at200r/min,the fermented broth had the highest prodigiosin content of11.56g/L.This study could provide a basis for the microbialproduction of prodigiosin from high-temperature rapeseed meal.
作者
陈新颜
操丽丽
杨培周
王康林
章华
操新民
郑志
姜绍通
CHEN Xinyan;CAO Lili;YANG Peizhou;WANG Kanglin;ZHANG Hua;CAO Xinmin;ZHENG Zhi;JIANG Shaotong(Anhui Key Laboratory of Intensive Processing of Agricultural Products, School of Food Science and Engineering,Hefei University of Technology, Hefei 230009;Pingguang Pharmaceutical Limited by Share Ltd., Hefei 231131, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第4期231-236,共6页
Food Science
基金
合肥工业大学大学生创新创业训练项目(201510359056)
安徽省科技攻关项目(1604a0702001)
关键词
黏质沙雷氏菌
灵菌红素
菜籽饼粕
响应面优化
发酵
Serratia marcescens
prodigiosin
rapeseed meal
response surface optimization
fermentation