摘要
目的探讨血管生成素-2(Ang-2)及其受体内皮细胞特异性酪氨酸激酶受体(Tie-2)在宫颈癌组织中发生、发展和临床病理学特征的关系。方法采用免疫组织化学、RT-PCR及Western blot分别检测Ang-2及Tie-2在60例正常宫颈及宫颈癌组织中的组织定位、基因差异表达及蛋白差异表达;采用Spearman法分析Ang-2及Tie-2的表达相关性。结果宫颈癌组织中Ang-2及Tie-2的阳性表达率明显高于正常宫颈组织,差异均有统计学意义(均P<0.05)。Ang-2及Tie-2在淋巴结发生转移或临床分期Ⅲ~Ⅳ级的患者中的表达明显高于未发生淋巴结转移或临床分期Ⅰ~Ⅱ级的患者(均P<0.05)。宫颈癌组织中Ang-2及Tie-2 m RNA及蛋白表达水平均明显高于正常宫颈组织,差异均有统计学意义(均P<0.01)。Tie-2与Ang-2在宫颈癌组织中的表达呈正相关(r=0.847 9,P<0.01)。结论 Ang-2及Tie-2的表达水平与宫颈癌临床分期和有无淋巴结转移密切相关,且Ang-2、Tie-2 m RNA及蛋白在宫颈癌组织中的表达明显高于正常宫颈组织,Ang-2及Tie-2可能在宫颈癌发生、发展过程中发挥重要作用。
Objective To investigate the expression of angiopoietin-2(Ang-2)and its receptor Tie-2(Tie-2)in cervicalcancer and their clinical significance.Methods The protein and mRNA expressions of Ang-2and Tie-2in60cervical cancertissue samples and60normal cervical tissue samples were detected by immunohistochemical technique,RT-PCR and Westernblot,respectively.The association between Ang-2,Tie-2expression and clinical features was analyzed by Spearman analysis.Results Ang-2and Tie-2were detected in the cytoplasm of cancer cells.The expression of Ang-2and Tie-2were significantlyhigher in cervical cancer tissues than that in normal tissues(all P<0.05).The expression of Ang-2and Tie-2was significantlyhigher in patients with lymph node metastasis and clinical stage III-IV than that in patients without lymph node metastasis andwith clinical state I-II(all P<0.05).Expression of Ang-2was positively correlated with expression of Tie-2(r=0.8479,P<0.01).Conclusion The expressions of Ang-2and Tie-2are up-regulated in cervical cancer,particularly in late stage cancer with lymphnode metastasis,which indicates that Ang-2and Tie-2may play an important role in the progress of cervical cancer.
出处
《浙江医学》
CAS
2017年第4期266-269,共4页
Zhejiang Medical Journal
基金
宁波市鄞州区农业与社会发展领域科技项目
