硫化氢对大鼠肝纤维化的抑制作用及机制研究

Inhibitory effect of hydrogen sulfide on hepatic fibrosis in rats and its mechanism

目的:探讨硫化氢对大鼠肝纤维化的抑制作用及作用机制。方法:选取27只雄性大鼠,将其随机分为三组,分别是对照组、空白对照组、观察组,每组9只。对对照组的大鼠腹腔注射四氯化碳,诱导大鼠肝纤维化;对观察组大鼠腹腔实施40%四氯化碳的基础上,还要在其腹腔注射硫化氢钠,每天注射1次,注射8周后停止。在实验结束后,检测大鼠的肝功能和纤维化指标数值,观察肝组织病理学变化,应用RT-PCR和Western印迹法检测肝组织转化生长因子TGF-β_1的表达。结果:与对照组相比较,观察组能降低血清丙氨酸氨基转换酶(ALT)、天门冬氨酸氨基转酶(AST)及透明质酸(HA)、层粘连蛋白(LN)、三星前胶原(PC)水平,升高白蛋白(ALB),改善肝功能等,大鼠整体的肝组织病理明显改善。结论:硫化氢对控制肝纤维化有非常好的效果,并且也能减少细胞外基质在肝脏中的沉积,能够对肝纤维化的进程有显著的延缓作用。

Objective:To explore the inhibitory effect of hydrogen sulfide on hepatic fibrosis in rats and its mechanism.Methods:27male rats were selected and randomly divided into three groups:the control group,blank control group and the observation group,with9rats in each group.Rats in the control group were injected intraperitoneally with carbon tetrachloride to induce hepatic fibrosis in rats;Rats in the observation group were injected intraperitoneally with sodium bisulfide on the basis of the injection of40%carbon tetrachloride,the injection was once every day and lasted8weeks.After the experiment,the liver function and fibrosis indexes of rats were detected and the pathological changes of liver were observed,the expression of transforming growth factor TGF-β1was detected by RT-PCR and Western blotting.Results:Compared with that of the control group,the observation group could decrease the level of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and hyaluronic acid(HA),laminin(LN)and PC;Albumin(ALB)and liver function were improved,and the liver pathological changes were significantly improved.Conclusion:Hydrogen sulfide had a very good effect on the control of liver fibrosis,and it could also reduce the deposition of extracellular matrix in the liver,which could significantly delay the process of liver fibrosis.