HPLC测定温阳振衰颗粒中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1含量

Content Determination of Ginsenoside Rg_1, Ginsenoside Re and Ginsenoside Rb_1 in Wenyang Zhenshuai Granules by HPLC

目的采用反相高效液相色谱法同时测定温阳振衰颗粒中人参皂Rg_1、人参皂苷Re、人参皂苷Rb_1的含量。方法采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%磷酸水,梯度洗脱(0~35 min,19%乙腈;35~58 min,19%~29%乙腈;58~70 min,28%乙腈;70~100 min,29%~40%乙腈),流速1.0 m L/min,检测波长203 nm,柱温35℃。结果人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1的进样量分别在0.22~2.2μg、0.22~2.2μg、0.26~2.6μg范围内与峰面积呈良好的线性关系,相关系数分别为0.999 8、0.999 8、0.999 1;人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1的平均回收率分别为98.04%、96.58%、96.75%。结论本方法准确、灵敏度高、重复性好,可作为温阳振衰颗粒的质量控制方法。

Objective To establish an HPLC method to determine the contents of ginsenoside Rg1,ginsenosideRe and ginsenoside Rb1in Wenyang Zhenshuai Granules.Methods The separation was preformed on Agilent TC-C18column(4.6mm×250mm,5μm).The mobile phase consisted of acetonitril-0.05%aqueous solution of phosphoricacid with gradient elution(0–35min,19%acetonitrile;35–58min,19%–29%acetonitrile;58–70min,28%acetonitrile;70–100min,29%–40%acetonitrile).The flow rate was1.0mL/min;the wavelength of detector was203nm;the temperature of column was35℃.Results The calibration curves showed good linearity in the range of0.22–2.2μg(ginsenoside Rg1,r=0.9998),0.22–2.2μg(ginsenoside Re,r=0.9998)and0.26–2.6μg(ginsenoside Rb1,r=0.9991),respectively.The average recoveries of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1were98.04%,96.58%and96.75%,respectively.Conclution The method is accurate,hypersensitized and reproducible,which can be appliedto the quality control of Wenyang Zhenshuai Granules.