重金属超富集植物东南景天毛状根的诱导

Induction of Hairy Roots in Heavy Metal Hyperaccumulator Sedum alfredii

为建立重金属超富集植物东南景天(Sedum alfredii)的毛状根诱导体系,采用发根农杆菌(Agrobacterium rhizogenes)A4侵染叶片,研究了预培养时间、侵染时间和共培养时间对毛状根诱导率的影响。结果表明,东南景天叶片外植体的预培养时间为48 h、农杆菌侵染时间为6 min、共培养时间为48 h是适宜的毛状根诱导时间,毛状根的诱导率可达85%。PCR检测表明诱导的毛状根中存在rol B基因片段。这是东南景天首次建立用发根农杆菌诱导毛状根体系。

In order to establish induction system of hairy roots of Sedum alfredii,the leaves infected by Agrobacterium rhizogenes A4as explants,the effect factors,such as pre-culture time,infection time andco-culture time,on induction rate of hairy roots were studied.The results showed that the hairy roots could beinduced after48h pre-culture,infection for6min and48h co-culture with the highest induction rate of85%.Furthermore,the rolB gene could be detected in induced hairy roots by PCR,which confirmed that the transgenic system of S.alfredii mediated by A.rhizogenes A4was successful.So,it was the first time for induction system establishment of hairy roots in S.alfredii with A.rhizogenes A4.