高效液相色谱法同时测定功能饮料中烟酰胺、咖啡因、维生素B_6、柠檬黄、胭脂红和苯甲酸的含量

Simultaneous Determination of Niacinamide, Caffeine, Vitamin B_6, Tartrazine, Carmine and Benzoic Acid in Functional Beverage by High Performance Liquid Chromatography

建立了一种同时快速检测功能饮料中烟酰胺、咖啡因、维生素B_6、柠檬黄、胭脂红和苯甲酸6种食品添加剂的高效液相色谱方法。6种食品添加剂的检出限分别为烟酰胺0.1 mg/L,咖啡因0.1 mg/L,维生素B6 0.2 mg/L,柠檬黄0.2 mg/L,胭脂红0.1 mg/L,苯甲酸0.1 mg/L,测定结果的相对标准偏差不大于4.57%(n=6),加标回收率在95.80%~113.68%之间。该方法满足GB/T 5009.197–2003,GB/T 23495–2009和SN/T 2105–2008对于上述6种食品添加剂检出限的要求。

An fast method was developed to simultaneously detect the content of niacinamide,caffeine,vitamin B6,tartrazine,carmine and benzoic acid in functional beverage by HPLC.The limits of detection(LOD)of niacinamide,caffeine,vitamin B6,tartrazine,carmine and benzoic acid were0.1,0.1,0.2,0.2,0.1,0.1mg/L,respectively.The relative standard deviations were not more than4.57%(n=6),and the recoveries ranged from95.80%to113.68%.The method was suitable for the demand of LOD of niacinamide,caffeine,vitamin B6,tartrazine,carmine and benzoic acid from GB/T5009.197–2003,GB/T23495–2009and SN/T2105–2008.