苏木素作为外源性波谱标志物进行肿瘤MRS分子成像的初步研究

Hematoxylin as an Exogenous MR Spectroscopic Biomarker in Tumor Molecular Imaging: A Pilot Study

目的探讨苏木素作为外源性波谱标志物进行肿瘤MRS分子成像的相关基础问题。方法用高场MR检测不同浓度苏木素溶液,分别用点解析波普法(Point Resolved Spectroscopy,PRESS)和激励回波获取法(Stimulated Echo Acquisition Mode,STEAM)法进行MRS成像,分析不同扫描方法和浓度苏木素MRS信号特点。结果无论PRESS和STEAM,苏木素均可检出特征性波谱谱线,峰位固定,但峰高和峰面积和浓度相关,浓度越高,谱线越清晰。结论苏木素有特征性MRS信号,有可能为肿瘤分子成像提供合适的外源性靶标物质。

Objective To discuss the basic problems of hematoxylin as an exogenous MR spectroscopic biomarker in tumor molecular imaging. Methods Different concentrations of hematoxylin solutions were performed high-field MR spectroscopy with the methods of Point Resolved Spectroscopy (PRESS) and Stimulated Echo Acquisition Mode (STEAM) respectively. Then the MR spectroscopy signal features of different concentrations of hematoxylin which was performed with different scanning methods were analyzed. Results The results showed that the specific MR spectroscopy signal profile of hematoxylin could be detected with both methods of PRESS and STEAM. The peak position was stable, while the peak height and peak area were related with the concentration of hematoxylin solution, the higher the concentration was, the more salient the specific MR spectroscopy signal profile was. Conclusion It was found that the hematoxylin has specific MR spectroscopic signal, which might make it a potential exogenous MR spectroscopic biomarker in tumor molecular imaging.