肿瘤特异性成像中固相萃取对Gd-DOTA-OCT小分子探针构建的影响

Efficacy of Brain Tumor Specific Imaging Solid Phase Extraction in the Construction of Gd-DOTA-OCT Small Molecular Imaging Probe

目的探讨"一步法"构建Gd-DOTA-OCT小分子MR探针,并用固相萃取(Solid Phase Extraction,SPE)对其进行分离。方法采用商品化DOTA-OCT直接用Gd Cl3螯合,并用SPE进行分离和提纯,对分子探针进行紫外分光光度检测、质谱检测和MR信号检测。结果"一步法"可以成功合成Gd-DOTA-OCT,并用SPE进行成功分离,经质谱检测与理论分子量相同,紫外分光光度法检测探针和MR信号检测证明分子探针完整。结论 Gd-DOTA-OCT小分子探针可以用一步法成功构建,并可用SPE方便地进行分离和纯化,为肿瘤特异性成像奠定基础。

Objective To discuss the effect of “one step method” on constructing GdTetraazacyclododecaneOctreotide(Gd-DOTA-OCT) small molecular MR imaging probe by adopting Solid Phase Extraction(SPE)method to isolate it from the mixture. Methods The commercial DOTAOCTwas chelated with GdCl3 directly, and the mixture was isolated and purified with SPE method, then all the isolated fractions were underwent ultraviolet spectrophotometric detection, mass spectrometric detection and MR signal detection respectively. Results GdDOTAOCTwas synthesized with “one step method” and isolated with SPE method successfully. The actual molecular weight of the probe detected by MALTITOF was consistent with the theoretic molecular weight, and the GdDOTAOCTMR probe wasintact after being verified by the ultraviolet spectrophotometric detection and MR signal detection.Conclusion GdDOTAOCTsmall molecular MR probe can be successfully constructed with “one step method” and can be isolated and purified with SPE method, which lays a foundation for brain tumor specific imaging.