孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响

Effect of Prepartum Supplementation of Pregnant Rats with Zinc Threoninate on the Growth Performance and Related Genes Expression of Their Offspring

为研究母体补充苏氨酸锌对子代生长水平的影响,实验将40只受孕SD大鼠随机分为5组,于孕鼠妊娠期第6~15天分别灌胃补充高、中、低剂量苏氨酸锌(灌胃剂量分别为250、500、1 000 mg/(kg·d)),孕鼠分娩后将仔鼠常规饲养,并测量1月龄仔鼠的生长指标和相关基因的表达。结果表明,苏氨酸锌各剂量组仔鼠体质量与对照组相比有显著提高(P<0.05);苏氨酸锌各剂量组与对照组相比较,其组织中锌转运体Zn T1基因的相对表达量都有显著提高(P<0.05);苏氨酸锌高剂量组与对照组相比较,其组织中胰岛素样生长因子IGF1基因相对表达量都有显著提高(P<0.05);且胰岛素样生长因子受体基因IGF1R的表达与IGF1的表达趋势基本保持一致。实验说明妊娠期补充苏氨酸锌能在一定程度上提升仔鼠的生长性能,能显著提高仔鼠锌转运体Zn T1、胰岛素样生长因子IGF1及其受体IGF1R基因表达水平。

The purpose of this study was to observe the effect of prepartum supplementation of zinc threoninate(Thr-Zn)for pregnant rats on the growth performance of their offspring.Forty pregnant SD rats were randomly divided into five groups.Thr-Zn at high,medium and low doses during the sixth to fifteenth day of pregnancy was orally administered to the three experimental groups of animals,respectively.After the newborn rats were routinely fed to one month of age,the growth indicators and the expression of growth-related genes were evaluated.Compared with the control group,body weights of the offspring rats from each dose group increased significantly(P<0.05).In addition,the mRNA expression levels ofzinc transporter1(ZnT1)and insulin-like growth factor1(IGF1)were increased significantly(P<0.05).The expression of insulin-like growth factor receptor(IGF1R)exhibited the same trend as IGF1.Therefore,the growth performance of offspring rats could be improved,and the mRNA expression levels of ZnT1,IGF1and IGF1R could be increased significantly by prepartum zinc supplementation.