包埋培养法筛选白酒窖泥中一株功能细菌

Screening of a Functional Bacterial Strain from Chinese Liquor Pit Mud by Microencapsulation Culture Method

采用微包埋培养法结合流式细胞分选技术,对白酒窖泥中的细菌进行分离和筛选,分离到95株细菌。经菌株生长实验从中筛选出8株生长较缓慢的菌株LS4、LS27、LS32、LS37、LS64、LS66、LS69’、LS85,经气相色谱分析,LS66菌株产白酒香味物质较为丰富,确定其为出发菌株。对其进行形态学观察、生理生化和16S r DNA鉴定,该菌株可能为一株窖泥未培养功能菌株uncultured bacterium clone N8-7,对该菌株的培养条件进行初步研究,菌株生长的最适温度、最适p H值和最佳发酵接种量分别为35℃、p H 6.5和4%。

A total of95bacterial strains were isolated from Chinese liquor pit mud by the microencapsulation culture methodthrough cell sorting using flow cytometry.Out of these isolates,the strains LS4,LS27,LS32,LS37,LS64,LS66,LS69’andLS85were selected for slow growth rates.Gas chromatography analysis demonstrated that LS66was determined as the bestone for the production of an abundance of aroma compounds in Chinese liquor.By morphological observation,physiologicaland biochemical tests and16S rDNA sequence analysis,it was tentatively identified as an uncultured functional bacterium,termed N8-7.The optimal culture temperature,pH and inoculums size for this strain were determined to be35℃,6.5and4%,respectively.