UPLC-MS/MS法测定动物源性食品中硝基呋喃代谢物残留量

Determination of nitrofuran metabolites residues in animal derived food by UPLC-MS/MS

本研究建立了动物源性食品中四种硝基呋喃代谢物的超高效液相色谱-串联质谱（UPLC-MS/MS）的检测方法。该方法对4种硝基呋喃代谢物的检出限均为0.25μg/kg，线性范围均为0.5～10.0μg/L，加标回收率为81.6%～97.3%，相对标准偏差均小于10%。该方法测定时间短、稳定性强、灵敏度高、重现性好，能满足畜禽肉中4种硝基呋喃代谢物残留检测的要求。

This study established the determination of nitrofuran metabolites residues in animal derivedfood by ultra pressure liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The detection limits of four kinds of nitrofuran metabolites were0.25μg/kg and the linear range was between0.5～10.0μg/L,the recovery rates were81.6%～97.3%,the RSD were less than10%.The method is rapid,simple,precise,sensitive and suitable for determination of nitrofuran metabolites residues in animal derived food.