LUNX与VEGFA基因联合检测对Ⅰ期非小细胞肺癌淋巴结微转移的临床意义

Expression of LUNX and VEGFA mRNA in lymph nodes of patients with stage I non-small-cell lung cancer and its clinical sigmficance

目的探讨人类肺组织特异性X蛋白(LUNX)与血管内皮生长因子A(VEGFA)基因联合检测对I期非小细胞肺癌(NSCLC)淋巴结微转移的临床意义。方法选择入院手术的39例I期NSCLC患者(淋巴结78枚)为实验组,同期入院的10例肺良性肿瘤患者(淋巴结19枚)为对照组,采用RT-PCR检测两组患者淋巴结LUNX、VEGFA m RNA表达,并分析其表达与NSCLC患者临床特征的相关性。结果实验组淋巴结LUNX、VEGFA m RNA阳性表达率为23.08%、19.23%,分别高于对照组的5.26%、0.00%(均P<0.05)。与常规病理检测比较,RT-PCR检测LUNX、VEGFA m RNA表达诊断NSCLC淋巴结微转移阳性率均较高(均P<0.05)。I期NSCLC患者淋巴结LUNX、VEGFA m RNA表达水平与肺癌病理类型、细胞分化程度、TNM分期均呈正相关(r=0.661、0.552和0.527,均P<0.05),与患者性别、年龄、吸烟史等未见相关(r=0.212、0.236和0.183,均P>0.05)。结论 LUNX、VEGF基因联合检测可辅助常规病理检测判断I期NSCLC淋巴结微转移,为临床诊治提供一定的帮助。

Objective To evaluate the expression of LUNX and VEGFA gene in lymph nodes of patients with stage I non-small cell lung cancer (NSCLC) and its clinical significance. Methods The expression of LUNX gene and VEGFA gene in tumor tissue and lymph nodes was detected by RT-PCR in 39 stage I NSCLC patients (cancer group) and 10 lung benign tumor patients (benign group). The association of LUNX and VEGFA expression with age, sex, smoking history and clinicopathological features of NSCLC patients was analyzed. Results The positive expression rates of LUNX mRNA and VEGFA mRNA in cancer group were 23.08% and 19.23%, which were significantly higher than those of benign group (5.26% and 〇.〇〇%, P<〇.〇5). The expression rates of LUNX mRNA and VEGFA mRNA in lymph nodes were significantly higher than metastasis rate detected by routine pathological examination (both P<〇.〇5). The expression of LUNX mRNA and VEGFA mRNA in lymph nodes was closely correlated with pathological type, cancer cell differentiation and clinic stage (r=〇.661, 0.552, 0.527, all P <〇.〇5), while not correlated with age,sex and smoking history (r=〇.212, 0.236, 0.183, all P >〇.〇5). Conclusion The expression of LUNX mRNA and VEGFA mRNA in the lymph nodes may be an indicator of micrometastases in patients with stage I NSCLC.