急性脑梗死患者循环血小板微颗粒和内皮细胞微颗粒水平的研究

Study of circulating platelet microparticles and endothelial microparticles levels in acute cerebral infarction patients

目的分析急性脑梗死(ACI)患者循环血小板微颗粒(PMP)及内皮细胞微颗粒(EMPs)水平。方法 300例ACI患者分为大面积梗死组72例和小面积梗死组228例,另取健康对照组60例。采集空腹外周静脉血,测定血浆PMP、EMPs及血清心型脂肪酸结合蛋白(H-FABP)、S-100β蛋白(S-100β)、神经元特异性烯醇化酶(NSE)、基质金属蛋白酶原(MMP-9)水平。分析PMP、EMPs与H-FABP、S-100β、NSE、MMP-9的相关性。结果大面积梗死组的血浆PMP和EMPs水平高于小面积脑梗死组,健康对照组最低,差异有统计学意义(P<0.05)。大面积梗死组的血清H-FABP、S-100β、NSE、MMP-9水平高于小面积脑梗死组,对照组最低,差异均有统计学意义(P<0.05)。ACI患者PMP和EMPs水平与H-FABP、S-100β、NSE、MMP-9水平均呈正相关(P<0.05)。结论高水平的PMP及EMPs可能参与了ACI的发生和发展,有望成为疾病诊断、病情评估和预后的新指标。

Objective To study was attempted to evaluate the expression levels of circulating platelet microparticles(PMP)and endothelial microparticles(EMPs)in acute cerebral infarction(ACI)patients.Method A total of300ACI patients have enrolled,including large area ACI group(n=72)and small area ACI group(n=228),meanwhile60healthy persons were selected as the control group.Collection fasting peripheral venousblood and detected the plasma levels of PMP and EMPs and the serum levels of H-FABP,S-100β,NSE,MMP-9.The relationship between PMP,EMPs and H-FABP,S-100β,NSE,MMP-9were observed.Results The plasma levels of PMP,EMPs and the serum levels of H-FABP,S-100β,NSE,MMP-9were all higher in large areaACI group than small area ACI group significantly,moreover,the control group were the lowest(P<0.05).There were positive relationship between levels of PMP,EMPs and levels of H-FABP,S-100β,NSE,MMP-9(P<0.05)Conclusion The high levels of PMP and EMPs may be involved in the development of ACI,which are expected to become new indexes in evaluation of disease,diagnosis and prognosis.