日本血吸虫成虫的非编码RNA高通量测序分析

Analysis of the non-coding RNA in adult Schistosoma japonicum by high-throughput sequencing

目的通过对日本血吸虫成虫做高通量测序分析,鉴定已知miRNA的表达水平,预测新miRNA的序列结构及生物学功能。方法首先提取日本血吸虫成虫miRNA进行深度测序,其次对miRNA做数据分析、统计长度分布与比对注释,鉴定已知miRNA表达水平,最后对发现的新miRNA序列进行结构和功能的预测。结果将构建文库中日本血吸虫表达的miRNA与最新Sanger miRBase数据库中的miRNA比对。结果显示,在鉴定的miRNA中,sja-mir-125b是表达量最高的miRNA,另外sjabantam,sja-mir-10,sja-mir-71a,sja-mir-36,sja-mir-61的表达量也较高,上述六种miRNA表达量占到已知miRNA表达量的94.6%。本研究发现了十种在日本血吸虫表达量最高的新miRNA,并对新miRNA进行序列及结构的预测。结论日本血吸虫成虫新miRNAs例如sja-mir-125b的发现将为深入研究其在血吸虫发育与致病的生物学功能打下基础。

Objective To predict the miRNA sequence an its biological function by determining the miRNA expression level in adult Schistosoma japonicum with high-throughput sequencing technique. Methods The miRNAs of Schistosoma japonicum were extracted and analyzed by high-throughput sequencing. Length distribution and expression levels of miRNAs were identified and annotated. Then the structure and expression of newly identified miRNA sequences were predicted with software tool, and aligned with Schistosoma japonicum miRNAs in library of latest Sanger miRBase database. Results In the Schistosoma japonicum miRNAs, sja-mir-125b was maximally expressed, followed by sja-bantam, sja-mir-10, sja-mir-71a, sja-mir-36 and sja-mir61, which accounted for 94.6% of the known miRNAs. Additionally, ten new miRNAs of Schistosoma japonicum were found, and their sequences and structures were further predicted. Conclusion Novel miRNAs, especially sja-mir-125b detected in our work may lay a foundation for following research in Schistosoma japonicum development and pathogenic.