期刊文献+

猕猴桃采后腐烂病原的鉴定 被引量:7

Identification of the pathogen associated with postharvest fruit rot of kiwifruit
下载PDF
导出
摘要 2015年9月,于湖南省浏阳果园内发现大量采摘后易腐烂的猕猴桃,腐烂部位有明显块状褐色病斑。对染病猕猴桃腐烂部位进行病原物的分离纯化、形态学观察和致病力验证、分子生物学方法进一步区分鉴定病原菌,结果表明,导致猕猴桃腐烂的病原菌为大豆拟茎点种腐病菌(Phomopsis longicolla)。 In September2015,post–harvest rot were found on a large number of kiwifruits in Hunan province of China which shows obvious brown disease spot.To figure the reason out,pathogen segregation and purification assay were conducted on the rotten parts of the kiwifruit.By morphological observation,pathogenicity verification,and molecular biological methods Phomopsis longicolla was finally identified as the cause of the kiwifruit rot.
作者 陆训 潘显婷 庞立 王仁才 周倩 LU Xun;PAN Xianting;PANG Li;WANG Rencai;ZHOU Qian(College of Plant Protection,Hunan Agricultural University, Changsha 410128, China;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Changsha 410128, China;College of Horticultural & Landscape, Hunan Agricultural University, Changsha 410128, China)
出处 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2017年第2期171-175,共5页 Journal of Hunan Agricultural University(Natural Sciences)
基金 湖南省教育厅高校科技创新平台项目(14K045)
关键词 猕猴桃 病原菌 拟茎点霉属 kiwifruits pathogen Phomopsis longicolla
  • 相关文献

参考文献2

二级参考文献17

  • 1朱衡,瞿峰,朱立煌.利用氯化苄提取适于分子生物学分析的真菌 DNA[J].真菌学报,1994,13(1):34-40. 被引量:305
  • 2萨姆布鲁克J 弗里奇EF 曼尼阿蒂斯T.分子克隆实验指南[M].北京:科学出版社,1999..
  • 3WEBER D J,TRONE E J.Lipid changes during the germination of teliospores of the common bunt fungus,Tilletia caries[J].Can J Bot,1980,58:2263-2268.
  • 4GANG D R,WEBER D J.Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores of Tilletia species[J].Biotechniques,1995,19 (1):92,94,96-97.
  • 5TA PICH,INGO SCHUBERT.Midiprep method for isolation of DNA from plants with a high content of polyphenolics[J].Nucleic Acids Research,1993,21(14):3328.
  • 6Maier W,Begerow D,Weiβ M,et al.Phylogeny of the rust fungi:an approach using nuclear large subunit ribosomal DNA sequences.Canadian Journal of Botany.2003,81:12 ~23.
  • 7White T J,Bruns T D,Lee S B,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.In:Innis M A,Gelfand D H,Sninsky J J,White T J(eds).PCR protocols:a guide to methods and applications.Academic Press.San Diego,California.1990.315 ~322.
  • 8Farr D F,Castlebury L A,Rossman A Y.Morphological and molecular characterization of Phomopsis vacciniiand additional isolates of Phomopsis from blueberry and cranberry in the eastern United States.Mycologia.2002,94:494 ~ 504.
  • 9Thompson J D,Higgins D G,Gibson J,et al.:improving the sensitivity of progressive multiple alignment through sequence weighting,position-specific gap penalties and weight matrix choice.Nucleic Acids Research.1994,22:4673 ~ 4680.
  • 10Thompson J D,Gibson T J,Plewniak F,et al.The CLUSTAL windows interface:flexble strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Research.1997,24:4876 ~ 4882.

共引文献13

同被引文献62

引证文献7

二级引证文献44

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部