大熊猫源石膏样小孢子菌培养条件的筛选

Culture conditions screening for Microsporum gypseum isolated from giant panda

为探明大熊猫源石膏样小孢子菌的最佳培养条件,在不同培养基、碳源、氮源、温度、pH等条件下培养石膏样小孢子菌,用十字交叉法测量菌落每天的生长情况。结果显示,石膏样小孢子菌在沙氏葡萄糖蛋白胨琼脂培养基上生长最好,在改良沙氏葡萄糖蛋白胨琼脂培养基、马铃薯葡萄糖琼脂培养基、高盐培养基上均生长良好,但在皮肤癣菌鉴定培养基和子囊孢子培养基上生长缓慢;5种碳源对菌株生长的影响不大,其中葡萄糖使该菌生长较好;在5种氮源培养基中,除了在以尿素为氮源的培养基中该菌株不能生长外,在以蛋白胨、硝酸钠、草酸铵、硫酸铵为碳源的培养基中该菌均能生长,且在以蛋白胨为氮源的培养基中生长最好;该菌株生长的最适培养温度为25℃,在15~35℃均可生长,在35℃时生长最差;在pH值为10时生长最好,在pH值为5、6、8、9时均能够良好生长,比在pH值为7时的生长更好,在pH值为4、11时的生长较差。

To study the optimal condition for the cultivation of Microsporum gypseum isolated from giant panda,different conditions including media,carbon sources,nitrogen sources,temperature and pH were selected as indicators to culture the strain,and the biological characteristics such as the color,morpholo and diameter of colony were observed using cross method every day.The results showed that the optimum medium for M.gypseum growth was SDA(sabouraud dextrose agar),it could also grow well in medium of M–SDA(modify sabouraud dextrose agar),PDA(potato dextrose agar)and TM(takashio mediun),while it grew slowly in medium of DTM(dermatophytes identification Medium)and AA(ascospore agar).Impacts of mediums from five kinds of carbon sources on the growth of M.gypseum have little difference,and among of them,the glucose was the great one.Apart from urea,M.gypseum could grow well in other four nitrogen–containing mediums and peptone was the optimum one.The optimum cultivation temperature was25℃,andM.gypseum could grow well in the temperature ranging from15to35℃,but its growth was the worst in35℃.The optimum pH for its growth was10,M.gypseum grew better in pH5,6,8and9than in pH7,but when the pH value was in4and11,the worst growth happened.