虎杖总蒽醌固体脂质纳米粒的制备工艺研究

Preparation of Total Anthraquinone of Polygonum Cuspidatum Solid Lipid Nanoparticles

以卵磷脂和硬脂酸为脂质材料,分别运用薄膜-超声分散法、乳化蒸发-低温固化法、溶剂-乳化挥发法、高压匀质法制备虎杖总蒽醌固体脂质纳米粒,以筛选虎杖总蒽醌固体脂质纳米粒的最佳制备工艺.采用透射电镜观察虎杖总蒽醌固体脂质纳米粒的形态,激光纳米粒度仪测定其粒径和Zeta电位,HPLC法测定药物包封率,并进行虎杖总蒽醌固体脂质纳米粒的初步稳定性考察.结果表明,运用高压匀质法制备虎杖总蒽醌固体脂质纳米粒为类圆球状,粒径较均匀.平均粒径为(100±21)nm,包封率为(87.0±0.69)%,平均Zeta电位为-66.3 mV,且在4℃条件下贮存3个月无明显变化,说明本实验工艺合理、可行.同时,制备的纳米粒大小均匀,且稳定性良好.

In order to select the best method for the total anthraquinone of Polygonum cuspidatum solid lipid nanoparticles(SLN).With lecithin and stearic acid as the matrix materials,the total anthraquinone of Polygonum cuspidatumsolid lipid nanoparticles was prepared respectively by using film-ultrasonic dispersion method,emulsion evaporationsolidificationat low temperature,solvent-emulsion evaporation method and high pressure homogenization technology.The morphology of the SLN was observed within a transmission electron microscope,the particle size,Zeta potential,drug entrapment efficiency and the primary stability were also determined or investigated.The results showed thatthe high pressure homogenization technology was chosen as the optimal preparation method of the total anthraquinoneof Polygonum cuspidatum solid lipid nanoparticles.As seen from the results,the SLN was spherical and uniform,the particle size,Zeta potential and encapsulation efficiency were(100±21)nm,-66.3mV and(79.3±0.69)%,respectively.There was no obvious change for the SLN when stored at4℃for three months.The conclusion was thatthe experiment process was reasonable and feasible,the solid lipid nanoparticles were uniform,and their stabilitywas good.