槌果藤乙醇提纯物保护酿酒酵母免受氧化损伤机制研究

The Alcoholic Extract of Capparis zeylanica Linn Protects against Oxidative Damage in Saccharomyces cerevisiae: An Investigation into the Mechanism

采用AB-8大孔树脂纯化槌果藤乙醇浸提物,得到槌果藤乙醇提纯物(alcoholic extract of Capparis zeylanicaLinn,AECZ)。体外1,1-二苯基-2-三硝基苯肼自由基和羟自由基清除实验表明AECZ具有较强的自由基清除能力。之后采用酿酒酵母BY4741及其基因缺失型(Ctt1、Sod1、Gsh1、Gtt1和Gtt2)探索AECZ对氧化应激条件下对酵母细胞的保护作用。结果表明AECZ能明显地提高酵母细胞对于H_2O_2、CCl_4和CdSO_4氧化应激的耐受性,同时能明显地降低应激状态下酵母的脂质过氧化和细胞内氧化水平。GTT1基因在AECZ减轻氧化应激下酵母损伤中发挥重要的作用。本实验验证了AECZ在细胞内具有显著的抗氧化活性,可为进一步开发利用槌果藤提供理论依据。

The crude alcoholic extract of Capparis zeylanica Linn(AECZ)was purified through AB-8macroporous resin.The in vitro tests indicated that AECZ had potent hydroxyl radical and(1,1-diphenyl-2-picrylhydrazyl)radical scavenging activities.The cytoprotection of AECZ in Saccharomyces cerevisiae BY4741and its homogenous strains deficient in antioxidant enzyme genes under oxidative stress induced by H2O2,CCl4and CdSO4was explored.Interestingly,AECZ could dramatically protect yeasts against oxidative stresses induced by H2O2,CCl4and CdSO4.Adaptive treatment with AECZ could reduce the level of intracellular lipid peroxidation and reactive oxygen species in yeasts under stress conditions.Moreover,the GTT1gene played an important role in alleviating oxidative damage in yeast cells.This study for the first time demonstrated the intracellular antioxidant activity of AECZ,which will provide theoretical evidence for further exploitation and utilization of Capparis zeylanica Linn.