神经生长因子促进大鼠胫骨骨折愈合的实验研究

Nerve growth factor promotes the bone healing on tibial fracture model of rats

目的观察神经生长因子对大鼠胫骨骨折愈合以及胰岛素样生长因子表达的影响。方法建立标准大鼠胫骨骨折模型,分别给予生理盐水和神经生长因子,术后1、2、4周分别进行X线骨痂评定、胫骨湿重称量以及半定量RT-PCR检测胰岛素样生长因子-1 mRNA的表达。结果术后4周时,神经生长因子组骨痂量(132.63±5.98)mm^3和胫骨湿重(0.98±0.12)g,对照组骨痂量(85.22±6.23)mm^3和胫骨湿重(0.84±0.04)g,NGF组显著多于对照组(P<0.05);神经生长因子组的胰岛素样生长因子-1 mRNA的表达术后1周时为(0.82±0.17),术后2周时为(0.54±0.08),对照组分别为(0.55±0.20)、(0.41±0.09)。NGF组显著高于对照组(P<0.05)。结论应用神经生长因子可以促进大鼠胫骨各组愈合,同时促进骨折愈合过程中IGF-1表达。

Objective To study the effect of nerve growth factor(NGF)on tibial fracture healing in rats,and the expression of insulin-like grouthfactor-1(IGF-1).Methods60SD rats were randomly divided into the control group and NGF group.All rats were established tibial fracture models,and were treated by normal saline(NS)or NGF respectively.Then the bony callus volume were evaluated by X-ray at1,2and4weeks after operation,and tibia wet weighing were weighed.The expression of IGF-1mRNA were assayed with semiquantitative RT-PCR too.Results In NGFgroup,bony callus volume is(132.63±5.98)mm3and tibia wet weighing is(0.98±0.12)g.In control group,bony callus volume is(85.22±6.23)mm3and tibia wet weighing is(0.84±0.04)g.NGF group were increased significantly at4weeks after operation,compared with the control group(P<0.05).The expression of IGF-1mRNA in NGF group,which is(0.82±0.17)at1week,(0.54±0.08)at2weeks,were increased significantly at1,2weeks after operation(P<0.05),compared with the control group,which is(0.55±0.20)at1week,(0.41±0.09)at2weeks.Conclusion The resultssuggest that NGF can stimulated bone healing,and promoted the expression of IGF-1in the fracture healing process.