摘要
目的分析肿瘤环境下人类脂肪间充质干细胞(AMSCs)的旁分泌特征变化与肿瘤细胞侵袭的关系。方法体外培养AMSCs,收集其上清液制备培养基培养HCT116细胞。利用Transwell培养板共培养AMSCs与HCT116细胞。通过检测穿透人工基底胶的能力对比两种培养条件下HCT116细胞侵袭能力的差异,同时采用酶联免疫吸附实验(ELISA)检测共培养后AMSCs旁分泌因子表达变化情况。结果根据检测穿透人工基底胶的能力结果显示,共培养的HCT116细胞侵袭能力明显强于AMSCs培养液培养的HCT116细胞。共培养条件下肿瘤环境影响了AMSCs旁分泌因子的表达,共培养条件下AMSCs细胞血管内皮生长因子C(VEGFC)、成纤维细胞生长因子10(FGF10)、肿瘤坏死因子-α(TNF-α)以及白细胞介素-10(IL-10)分泌量明显高于细胞培养,差异均具有统计学意义(t=8.692、7.593、7.298、8.356,P<0.05)。结论肿瘤环境下会影响AMSCs旁分泌因子的表达,增加HCT116细胞侵袭能力。
Objective To analyze the relationship between paracrine characteristics changes of human adipose mesenchymal stem cells(AMSCs)and the tumor cells invasion in tumor environment.Methods AMSCs were cultured in vitro,and their supernatant was collected for preparation of medium HCT116cells.AMSCs and HCT116cells were co-cultured in Transwell culture palate.Difference of HCT116cells invasion ability between two culture conditions by detecting the ability of penetrating artificial base glue,and changes of AMSCs paracrine factor expression were detected by enzyme-linked immunosorbent assay(ELISA).Results Examination results of penetrating artificial base glue showed that HCT116cells of co-culture had obviously stronger invasion ability than HCT116cells cultured with AMSCs culture solution.Tumor environment of co-cultrure influenced AMSCsparacrine factor expression,and co-culture had obviously higher AMSCs cells vascular endothelial growth factorC(VEGFC),fibroblast growth factors10(FGF10),tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)secretion levels than cells culture,and their difference had statistical significance(t=8.692,7.593,7.298,8.356,P<0.05).Conclusion Tumor environment can influence AMSCs paracrine factor expression,and increase invasion ability of HCT116cells.
作者
彭大颖
吴迪
PENG Da-ying;WU Di(Department of Pathology,Liaoning Province Panjin City Liaohe Oil Field General Hospital, Panjin 124000, China)
出处
《中国现代药物应用》
2017年第7期60-62,共3页
Chinese Journal of Modern Drug Application
关键词
肿瘤环境
人类脂肪间充质干细胞
旁分泌特征
肿瘤细胞侵袭能力
Tumor environment
Human adipose mesenchymal stem cells
Paracrine characteristics
Tumor cells invasion ability