混合大孔树脂纯化海芦笋总黄酮工艺的研究

Purification of total flavonoids from Salicornia bigelovii Torr. usingmixed macroporous resin

以黄酮的吸附率、解吸率和回收率作为主要评价指标,对海芦笋(Salicornia bigelovii Torr.)总黄酮的纯化工艺进行了研究。结果表明,在5种大孔树脂中选取D101型、HPD600型两种树脂混合用于纯化,最佳混合比例为3︰2。混合树脂对海芦笋黄酮的最佳纯化工艺参数为:黄酮浓度为0.5 g/L时,pH为5的上样液以1.0 m L/min的上样流速进行吸附,上样量最大为27BV。待吸附完全,用4BV蒸馏水冲洗混合树脂柱后,用8BV60%的乙醇溶液进行洗脱,洗脱流速0.5 m L/min。采用该工艺纯化后,海芦笋黄酮的纯度提高至45.91%±0.44%,黄酮回收率为86.74%±0.46%,D101、HPD600混合树脂对海芦笋黄酮纯化效果良好,可用于海芦笋总黄酮的纯化。

Westudied the purification technology of total flavonoids from Salicornia bigelovii Torr.using adsorption,desorption,and recovery rates of flavonoids as the evaluationindices.D101and HPD600were selected from five types ofmacroporous resin for purification and were mixed in a ratio of3︰2forpurification.We showed that the optimal purification conditions includeflavonoids concentration of sample(0.5mg/mL),pH value(5),the flow rate(1mL/min),and the maximum sample volume(27BV).After completeadsorption,themacroporousresin column was washed with4BV distilled water with a flow rate of2mL/min and eluted with8BV60%alcohol and a flow rate of0.5mL/min.We found that the purity of the sample increased to45.91%±0.44%after purification,with a recovery rate of86.74%±0.46%.Thus,the mixed resin improves purification outcome,and thistechnology can also be applied tothe purification oftotal flavonoids from Salicornia bigelovii Torr.