山东省渤海海洋保护区典型海域表层海水微生物群落多样性分析

Analysis of microbial community diversity in the Bohai Sea marine protected areas of the Shandong Province

海洋微生物在生态系统循环中发挥重要作用。为系统了解山东省渤海海洋保护区表层海水微生物群落多样性,作者利用Illumina高通量测序及生物信息学分析技术研究了12个水体样本的菌群构成和多样性特征。实验结果表明,山东渤海海洋保护区典型海域表层海水微生物群落丰富,12个水体样品OTU分属于25门、56纲、103目、163科、227属;门、纲、目、科、属分类等级上的优势类群分别为变形菌门(Proteobacteria)(57.95%)、α-变形菌纲(Alphaproteobacteria)(21.88%)、黄杆菌目(Flavobacteriales)(4.31%)、红细菌科(Rhodobacteraceae)(10.68%)、Candidatus portiera(7.94%);保护区内表层海水微生物具有其独特的菌群特征和多样性。本研究结果为进一步认识海洋微生物多样性与海洋生态环境保护的关系奠定了基础。

Marine microbes play an essential role in marine ecosystems.To systematically understand microbial community diversity in the Bohai Sea marine protected areas of the Shandong Province,we investigated the microbial community composition and diversity of12water samples using the Illumina high-throughput sequencing and bioinformatics analysis technology.Results for the12sampling points showed that the number of relative microbial groups included25phyla,56classes,103orders,163families,and227genera.Dominant groups at the phylum,class,order,family and genus classification levels were Proteobacteria(57.95%),Alphaproteobacteria(21.88%),Flavobacteriales(14.31%),Rhodobacteraceae(10.68%),and Candidatus portiera(7.94%),respectively.These findings suggest that Bohai Sea marine protected areas in the Shandong Province brine have high levels of microbial diversity.This diversity provides basic information and knowledge to further understand the relationship between marine microbial diversity and protection of oceanic environment ecology.