响应面法优化浒苔半纤维素的提取条件及其组分分析

Optimization of hemicellulose extractionfrom Enteromorpha prolifera using response surface method and component analysis

以浒苔(Enteromorpha prolifera)为原料,采用响应面法优化碱性过氧化氢提取半纤维素的条件。在单因素实验的基础上,运用Box-Benhnken中心组合原理,设计四因素三水平实验,确定半纤维素最佳提取条件。采用红外光谱(FT-IR)和离子色谱(IC)分析浒苔半纤维素组分。结果表明,各因素对浒苔半纤维的提取率的影响为:时间>温度>料液比>氢氧化钠浓度。碱性过氧化氢法提取半纤维素的最佳条件为:氢氧化钠浓度为4.8 g/L,提取温度为92℃,提取时间为5 h,料液比1︰70,半纤维素的提取率为56.75%,与预测值57.30%基本一致。FT-IR结果显示,浒苔半纤维素红外吸收光谱中含有明显的木糖和阿拉伯糖的特征吸收峰,为吡喃环结构,由β-糖苷键连接。离子色谱结果表明,浒苔半纤维素主要由木糖和阿拉伯糖组成,含有少量葡萄糖和半乳糖。木糖含量为54.4%,其次为阿拉伯糖,含量为15.3%,葡萄糖和半乳糖含量分别为5.6%和2.7%。

We optimizedalkaline hydrogen peroxide extraction conditions of HC in Ep by RSM usingBox–Behnken central composite principle to design experimentswith fourfactorsand threelevels.HC components wereanalyzed usingFT-IR and IC.The results showed that the followingfactors influencethe extraction amount of HC in that order:time>temperature>ratio of Ep to solvent>concentration of sodium hydroxide.The optimal extraction conditions included a solid to liquid ratio of1︰70,sodium hydroxide concentration of4.8g/L,temperature of92℃),and extraction time of5h.The extraction rate wasobserved to be56.75%,consistentwith the predicated value of57.30%.FT-IR results showed that HC contains xylose and arabinose absorption peaks and pyran ring structure connectedby beta-glycosidic bond.IC results showed that HC was mainly comprisedxylose(54.4%),arabinose(15.3%),glucose(5.6%),and galactose(2.7%).