茉莉酸甲酯和水杨酸对坛紫菜生长与抗逆性的影响

Effects of methyl jasmonate and salicylic acid on the growth and stress resistance of Pyropia haitanensis

为了探讨植物激素在藻类生理与抗逆方面的作用,本文比较了不同浓度的茉莉酸甲酯(MJ)和水杨酸(SA)对坛紫菜(Pyropia haitanensis)生长、藻胆蛋白、可溶性蛋白、脯氨酸含量和叶绿素荧光参数的影响。结果显示100?mol/L SA的添加可使坛紫菜生长率达到对照组的1.57倍,而MJ对藻的生长影响不显著。50?mol/L MJ和100?mol/L SA显著促进了坛紫菜藻胆蛋白和可溶性蛋白的积累。50?mol/L MJ和100?mol/L SA添加后在48 h脯氨酸含量分别为对照组的2.67倍和1.75倍。25~50?mol/L MJ对坛紫菜的Fv/Fm和Y(II)影响不显著,而100?mol/L SA则可以提高这两个叶绿素荧光参数。该研究表明适当浓度的MJ和SA可以促进坛紫菜生长,提高其光合能力,在一定程度上增强坛紫菜的抗逆性。

To explore the effects of plant hormones on algal physiology and stress resistance,we compared the effects of different methyl jasmonate(MJ)and salicylic acid(SA)concentrationson the growth rate,phycobiliprotein content,soluble protein accumulation,proline content,and chlorophyll fluorescence parametersin Pyropia haitanensis.Results revealed that the growth rate of P.haitanensis in the100?mol/L SA treatment groups increased1.57folds compared with that in the untreated control group.MJ treatment had no significant effect on the algal growth.We demonstrated that treatment with50?mol/L MJ and100?mol/L SA could significantly improve the algal phycobiliprotein content and soluble protein accumulation.Compared with the untreated control,proline content increased by2.67and1.75folds at48h when treated with50?mol/L MJ and100?mol/L SA,respectively.While the treatment with25~50?mol/L MJ had no significant effect on Fv/Fm and Y(II),treatment with100?mol/LSA improved both the two chlorophyll fluorescence parameters of P.haitanensis.These results indicate that treatment with MJ and SA at appropriate dosages was beneficial to the growth,photosynthetic capacity,and stress resistance ability of P.haitanensis.