纤溶酶产生菌的鉴定及产酶优化

Identification of fibrinolytic enzyme-producing strain and optimization of producing enzyme conditions

目的对纤溶酶产生菌——LZ-01菌株进行种属鉴定并优化其产酶能力。方法对LZ-01菌株的形态特征进行观察,并进行生理生化试验和phoR基因的序列相似性分析以确定菌株种属,通过单因素试验考察碳源、氮源等对菌株发酵产酶活力的影响,采用正交实验确定菌株最佳产酶工艺条件。结果经鉴定,LZ-01菌株与解淀粉芽孢杆菌标准菌株FZ42的相似度达99.78%,鉴定LZ-01菌株为解淀粉芽孢杆菌。经工艺优化后的酶活力是未经优化酶活力的3.63倍。结论本研究为进一步开发纤溶酶制剂溶栓药物提供了一定的理论依据。

Objective To identify fibrinolytic enzyme-producing strain(LZ-01)and optimize producing enzyme conditions.Methods The morphological characteristics of LZ-01were observed.Physiological and biochemical tests and the sequence similarity analysis of phoR gene were adopted to determine the strain.Through single factor experiment,the influence of carbon source and nitrogen source on enzyme activity was investigated,and the optimal conditions of enzyme production were determined by orthogonal experiment.Results After the identification,the similarity of this strain and Bacillus amyloliquefaciens standard strain FZ42was99.78%.So,the strain was identified as Bacillus amyloliquefaciens.After optimizing,the producing enzyme activity increased by3.63times.Conclusions This study provides a theoretical basis for the further development of fibrinolytic drugs.