抗生素对厌氧氨氧化颗粒污泥脱氮性能的影响

Effect of antibiotics on the nitrogen removal performance of anammox granules

通过血清瓶批试实验,研究了3种具有不同抑制机理的抗生素(青霉素G钠、土霉素盐酸盐和硫酸多粘菌素E)对厌氧氨氧化颗粒污泥脱氮性能的短期抑制特性.不添加抗生素时,厌氧氨氧化颗粒污泥的NH_4^+-N和NO_2^--N降解速率分别为0.252,0.375kg N/(kg VSS·d).浓度为3000mg/L的青霉素G钠对厌氧氨氧化颗粒污泥的活性抑制作用较小.土霉素盐酸盐和硫酸多粘菌素E对厌氧氨氧化颗粒污泥的活性抑制作用较强.土霉素盐酸盐浓度分别为50,100,150,200,400mg/L实验组的NH_4^+-N降解速率分别为0.250,0.237,0.200,0.117,0.062kg N/(kg VSS·d);N0_2^--N降解速率分别为0.324,0.304,0.296,0.244,0.069kg N/(kg VSS·d).硫酸多粘菌素E浓度分别为30,70,90,100,300mg/L实验组的NH_4^+-N降解速率分别为0.230,0.134,0.094,0.022,0.007kg N/(kg VSS.d);N0_2^--N降解速率分别为0.351,0.203,0.133,0.039,0.004kg N/(kg VSS·d).青霉素G钠、土霉素盐酸盐和硫酸多粘菌素E对厌氧氨氧化颗粒污泥活性的抑制作用依次增强.

The inhibition effect of three different kinds of antibiotics on the nitrogen removal performance of anammox granules was investigated by serum bottle batch tests.The antibiotics used in the experiment were penicillin G sodium,oxytetracycline HCl and polymyxin E sulphate,which performed different inhibition mechanisms.As a result,when the batch test was conducted without the addition of antibiotic,the NH4+-N and NO2--N removal rates of the anammox granules were0.252and0.375kg N/(kg VSS·d),respectively.Besides,the anammox activity of the granules was not inhibited with the penicillin G sodium concentration of3000mg/L.However,the anammox activity of the granules was largely inhibited by oxytetracycline HCl and polymyxin E sulphate.With the oxytetracycline HCl concentrations of50,100,150,200and400mg/L,the NH4+-N removal rates were0.250,0.237,0.200,0.117and0.062kg N/(kg VSS·d),respectively;The NO2--N removal rates were0.324,0.304,0.296,0.244and0.069kg N/(kg VSS·d),respectively.Meanwhile,with the polymyxin E sulphate concentrations of30,70,90,100and300mg/L,the NH4+-N removal rates were0.230,0.134,0.094,0.022and0.007kg N/(kg VSS·d),respectively;The NO2--N removal rates were0.351,0.203,0.133,0.039and0.004kg N/(kg VSS·d),respectively.The inhibition effects of the three kinds of antibiotics were in the order as follows:polymyxin E sulphate>oxytetracycline HCl>penicillin G sodium.