Flot-2高表达促进食管鳞状细胞癌的生长和侵袭

High expression of Flot-2 promotes the development and invasion of esophageal squamous cell carcinoma

目的探究flotillin-2(Flot-2)在人食管鳞状细胞癌中的表达及基本功能。方法利用免疫组织化学技术检测76例人食管鳞状细胞癌组织及相应癌旁组织中Flot-2的免疫反应阳性水平,并分析其与食管鳞状细胞癌的相关性。以小分子si RNA降低人食管鳞状细胞癌细胞系KYSE150细胞中Flot-2表达后,利用CKK-8及Transwell小室实验,检测细胞生长和侵袭能力的变化。结果 Flot-2在人食管鳞状细胞癌组织中免疫反应性明显升高,其在转移组织中的免疫反应水平显著高于非转移组织。敲低Flot-2表达后,KYSE150细胞的生长和侵袭能力明显降低。结论 Flot-2能促进细胞的生长和侵袭,参与人食管鳞状细胞癌的发生与转移过程。

Objective To explore the expression and functions of flotillin-2(Flot-2)in human esophageal squamous cell carcinoma(ESCC).Methods The immunoreactivity of Flot-2in the cancerous and adjacent tissue from76ESCC cases was detected by immunohistochemistry,and its correlation with ESCC was analyzed.Small RNA interference(siRNA)was applied to reduce the expression of Flot-2in KYSE150ESCC cells,after which the proliferation and invasion abilities of KYSE150cells were examined by CCK-8and Transwell tests.Results Flot-2immunoreactivity was significantly higher in ESCC tissue than in adjacent tissue,and in metastatic ESCC than in non-metastatic ESCC.The proliferation and invasion abilities of KYSE150cells were obviously inhibited after Flot-2knockdown.Conclusion Flot-2plays a role in the development and metastasis of ESCC by promoting cell proliferation and invasion.