TNF-α、IL-6分子基因多态性与种植体周围炎相关性分析

Investigation on the relationship between gene polymorphisms of TNF-α,IL-6 and peri-implantitis

目的研究TNF-α和IL-6基因多态性与种植体周围炎是否具有相关性。方法选择90例种植体周围炎患者、40例慢性牙周炎患者及95例牙周健康者,双侧颊粘膜上取脱落的上皮细胞,应用聚合酶链反应-限制性内切酶片段长度多态性分析方法研究3组TNF-α、IL-6的基因型及等位基因分布特点,并探讨其与种植体周围炎的相关性。结果3组中IL-6-572位点等位基因的频率无明显差异(P>0.05);种植体周围炎组与慢性牙周炎组中TNF-α-308位点,G/G基因型的频率高于牙周健康组(P<0.05)。结论 IL-6-572的分子基因多态性与种植体周围炎无关联。TNF-α-308G/G分子基因多态性与种植体周围炎的发生有密切关系,可能是种植体周围炎的生物标记物。

Objective To determine whether TNF-αand IL-6were related with peri-implantitis at genetic level.Methods The genotype and allele distributions of TNF-αand IL-6involving95healthy cases,90patients with peri-implantitis and40patients with chronic-periodontitis were analyzed by PCR-restriction fragment length polymorphism.Results There was no significant difference among genotype G/G,C/G,C/C in IL-6-572of three groups.The genotype G/G in TNF-α-308increased in peri-implantitis and chronic-periodontitis groups in comparison with healthy group.Conclusion There is no association between IL-6-572single nucleotide polymorphisms and peri-implantitis.However,TNF-α-308G/G polymorphisms are associated with peri-implantitis and may present biomarker for peri-implantitis.