云南省边境地区口蹄疫抗体检测

Antibody Detection of Foot-and-mouth Disease in Border Areas of Yunnan Province

应用口蹄疫O、A、Asia-1型cELISA抗体检测试剂盒和口蹄疫非结构蛋白抗体单抗阻断ELISA试剂盒,对采集自云南省10个边境县的牛和猪血清进行检测。检测结果显示,牛口蹄疫O、A、Asia-1型抗体阳性率分别在9.23%~37.75%、3.00%~23.57%、9.75%~48.67%之间,阳性率偏低,并且地区间差异较大。非结构蛋白抗体阳性率在14.16%~51.00%之间,其中勐海为51.00%、芒市为48.96%,动物带毒风险较大。境内牛与境外入境牛口蹄疫O、A、Asia-1型抗体阳性率基本相当,非结构蛋白抗体阳性率略高于境内牛。由于入境牛免疫率较低,须加大对入境牛的监控。黄牛和水牛口蹄疫O、A、Asia-1型抗体阳性率基本一致,但黄牛非结构蛋白抗体阳性率为36.47%,水牛为26.86%,黄牛阳性率显著高于水牛,这可能是因为云南省边境地区的黄牛主要来自印度、孟加拉国,经缅甸进入我国。猪口蹄疫血清抗体阳性率较低,除芒市O型口蹄疫抗体阳性率达到81.00%外,其余均在0~6.00%之间,这可能与云南省近年来大量免疫合成肽疫苗有关。建议加强云南省边境地区的口蹄疫防控工作,提高口蹄疫免疫密度和抗体水平,严控边境地区口蹄疫易感动物的无序流动。

By using cELISA kits to detect antibodies against foot-and-mouth disease(FMD)type O,A,Asia-1,as well as the monoclonal antibody blocking ELISA kits to detect non-structural protein of FMDV,serum samples of bovine and swine that were collected from10border counties of Yunnan province were tested.Results showed that the FMD type O,A,Asia-1antibody positive rates of cattle were9.23%~9.23%,3.00%~23.57%,23.57%~48.67%,respectively,showing low levels,and the regional differences were relatively large.On the other side,the antibody positive rates of nonstructural protein were in the range of14.16%~51.00%,specifically,the rate in Menghai was51.00%,in Mangshi was48.96%.Besides,a high risk of animals carrying FMDV was observed.As to the cattle both in and outside borders,the antibody levels against type O,A,Asia-1were basically the same,while antibodies against nonstructural protein were higher in cattle outside the borders.Given the immunization rate of entry cattle was relatively low,monitoring for these cattle should be strengthened.Antibody positive rates of type O,A,Asia-1between cattle and buffaloes also showed basic consistent results.Unlike that,the antibody positive rate of nonstructural protein in battle was36.47%,while in buffalo it was26.86%.The reason caused this significant difference may related to the fact that cattle in Yunnan border areas were mostly transported from India and Bangladesh via Myanma.Antibody positive rate of FMD of swine was low,ranges of0~6.00%were detected in most counties except a rate of81.00%against type O of FMD in Mang city,immunization with synthetic peptide vaccines in great quantities may be responsible for these results.At the end,suggestions were put forward,including strengthening FMD prevention and control,intensifying vaccination and enhancing the antibody levels,as well as strictly controlling the unordered flow of susceptible bovines in border areas.