自噬相关蛋白PTEN、MAP1LC3在舌癌中的表达与意义

Expression of autophagy-related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma

目的探讨自噬相关蛋白人第10号染色体缺失的磷酸酶及张力蛋白同源基因(phosphatase and tensinhomology deleted on chromosome ten,PTEN)、微管相关蛋白1轻链3(microtubule-associated protein 1lightchain 3,MAP1LC3)在舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)、癌前病变及癌旁组织中的表达及相关性,探讨其与TSCC发生、发展及转移的关系。方法采用免疫组化SP法检测46例TSCC手术标本及13例癌前病变、13例癌旁组织标本中PTEN、MAP1LC3的蛋白表达,分析其与临床病理特征间的关系。结果 PTEN(X^2=9.905,P=0.007)、MAP1LC3(X^2=9.577,P=0.008)在TSCC组织、癌前病变、癌旁组织中的蛋白表达差异有统计学意义。在TSCC中,PTEN蛋白的阳性表达率(34.78%)明显低于癌旁组织(69.23%,X^2=4.926,P=0.026)及癌前病变组织(76.92%,X^2=7.302,P=0.007);MAP1LC3蛋白的阳性表达率(28.26%)明显低于癌旁组织(61.53%,X^2=4.896,P=0.027)及癌前病变组织(69.23%,X^2=7.275,P=0.007),差异均有统计学意义。PTEN与MAP1LC3蛋白表达与患者年龄、性别、吸烟、饮酒、细胞分化无关(P>0.05)。颈淋巴结从无转移到转移以及临床分期越晚,PTEN蛋白及MAP1LC3蛋白的阳性表达率降低,差异有统计学意义(P<0.05)。PTEN和MAP1LC3两种蛋白在复发组中阳性表达率皆较低,复发组与非复发组PTEN的蛋白表达差异有统计学意义(X^2=4.269,P=0.039),而复发组与非复发组MAP1LC3的蛋白表达差异尚无统计学意义(X^2=0.343,P=0.453)。PTEN与MAP1LC3蛋白阳性表达呈正相关。结论自噬相关蛋白PTEN与MAP1LC3的低表达与TSCC的临床病理特征相关,对TSCC的诊断与预后有一定参考价值。

Objective This study aims to evaluate the expression of autophagy?related protein cancer phosphatase and tensinhomology deleted on chromosome ten(PTEN)and microtubule?associated protein1light chain3(MAP1LC3)and investigate its significance in tongue squamous cell carcinoma(TSCC).Methods About46TSCC samples,13cases of precancerous lesion and13cases of adjacent tissues were enrolled.Immunohistochemistry were performed to examine the expression of PTEN,MAP1LC3.Statistical analyses were carried out to assess the associations among clinic pathologic parameters.Results The positive expression rate of PTEN(34.78%)in TSCC was significantly lower than that in adjacent tissues(69.23%,c2=4.926,P=0.026)and precancerous lesions(76.92%,c2=7.302,P=0.007).The positive expression rate of MAP1LC3(28.26%)in TSCC was significantly lower than that of adjacent tissues(61.53%,c2=4.896,P=0.027)and precancerous lesions(69.23%,c2=7.275,P=0.007),the difference was statistically significant.The expression of PTEN and MAP1LC3was not correlated with age,sex,smoking,alcohol consumption and cell differentiation(P>0.05).There was a significant difference in the expression of PTEN and MAP1LC3in subgroup with different clinical stage and in subgroup with or without lymph node metastasis(P<0.05).There is a significant difference in the tumor recurrence between PTEN protein expression positive subgroup and PTEN protein expression negative subgroup(c2=4.629,P=0.039),and there was no significant difference in the tumor recurrence between MAP1LC3protein expression positive subgroup and MAP1LC3protein expression negative subgroup(c2=0.343,P=0.453).There was a positive correlation between PTEN and MAP1LC3.Conclusion The expression of PTEN and MAP1LC3suggested that autophagy associated proteins play a pivotal role in the progression,diagnosis and prognosis of TSCC.Down?regulation of PTEN and MAP1LC3was observed in TSCC.