黄芩苷对缺血性脑损伤雄性大鼠脑保护作用的机制研究

Study on Mechanism of Protective Effects of Baicalin on Male Rats with Ischemic Brain Injury

目的观察黄芩苷对缺血性脑损伤雄性大鼠的脑保护作用及其对大鼠血清孕酮水平的影响,探讨其可能的作用机制。方法采用成年SD雄性大鼠制造永久性左侧大脑中动脉梗阻模型,根据神经功能评分,分为模型组、黄芩苷组和抑制剂组,假手术组不插入拴线。造模后于不同时间点进行神经功能评分及双前肢抓力测定,计算抓力下降率;ELISA检测血清孕酮和促肾上腺皮质激素(ACTH)含量。结果与假手术组比较,模型组大鼠神经功能受损,双前肢抓力显著减小。治疗7 d后,与模型组比较,黄芩苷组神经功能评分降低,双前肢抓力恢复,抓力下降率减小(P<0.05);与黄芩苷组比较,抑制剂组黄芩苷神经功能保护作用降低(P<0.05)。治疗7 d后,与模型组比较,黄芩苷组血清孕酮水平显著升高(P<0.01),ACTH水平有升高趋势;与黄芩苷组比较,抑制剂组血清孕酮和ACTH水平均降低(P<0.05)。结论黄芩苷对缺血性脑损伤雄性大鼠的脑保护作用可能与其调控孕酮生成有关。

Objective To investigate the productive effects of baicalin on the male rats with ischemic brain injuryand its effects on serum progesterone level in rats;To explore the possible mechanism of baicalin in brain protection.Methods Adult SD male rats were used to create a permanent left middle cerebral artery occlusion model.The ratswere evenly divided into model group,baicalin group,inhibitor group,and sham-operation group(without insertedinto the intraluminal thread)according to the neurological function scores.At different time points after modeling,theneurological function scores and the grip strength of double foreleg were measured,and the reduction rate of gripstrength was calculated.Serum progesterone and adrenocorticotrophic hormone(ACTH)were detected by ELISA.Results Compared with the sham-operation group,the neurological function of rats in the model group was impaired,the grip strength of double foreleg was significantly reduced.7days after treatment,compared with the model group,the neurological function score of baicalin group was lowered,grip strength of double foreleg was recovered,reduction rate of grip strength was reduced(P<0.05);compared with the baicalin group,protective effects of baicalinon neurological function was lowered in inhibitor group(P<0.05).7days after treatment,compared with the modelgroup,the serum progesterone level in baicalin group was significantly higher(P<0.01),and ACTH level showed an increasing trend;compared with the baicalin group,serum progesterone and ACTH levels in the inhibitor groupdecreased(P<0.05).Conclusion The protective effects of baicalin on the male rats with ischemic brain injury may berelated to the regulation of progesterone.