HPLC测定不同产地北豆根中蝙蝠葛碱含量

Content Determination of Dauricine in Menispermi Rhizoma from Different Producing Areas by HPLC

目的建立不同产地北豆根中蝙蝠葛碱含量测定的HPLC方法。方法以C18为色谱柱填充剂,以乙腈-水-三乙胺(45∶55∶0.1)为流动相,检测波长为284 nm,流速为1.0 m L/min,柱温为30℃,建立8批不同来源的北豆根HPLC图。结果建立了检测北豆根的HPLC条件,在20~100μg/m L范围内,对照品进样量与峰面积呈良好的线性关系(r=0.999 5),蝙蝠葛碱的平均回收率为100.30%,RSD=1.000%。不同来源的北豆根中蝙蝠葛碱含量不同。结论本研究建立的HPLC方法灵敏,准确度、精密度、重复性好,操作简便,可作为北豆根中蝙蝠葛碱含量的检测方法。

Objective To establish an HPLC method to determine the contents of dauricine in MenispermiRhizoma from different producing areas.Methods C18was set as chromatographic column filler,with acetonitrilewater-triethylamine(45:55:0.1)as the mobile phase,284nm as the ultraviolet wavelength detection,1mL/min as theflow rate,30℃as the column temperature.HPLC chromatograms of eight different batches of Menispermi Rhizomawere established.Results HPLC testing conditions of Menispermi Rhizoma was established.Within20–100μg/mL,there was a good linear relationship between the injection volume of the reference substance and the peak area(r=0.9995).The average recovery of dauricine was100.30%,RSD=1.000%.The contents of dauricine in MenispermiRhizoma from different producing areas were different.Conclusion The HPLC method is with sensitivity,accuracy,precision,good reproducibility and simple operation,which can be used as detection method to determine the contentof dauricine in Menispermi Rhizoma.