聚多巴胺辅助磁微球固定β-葡萄糖苷酶的制备与应用

Polydopamine-Assisted Preparation and Application of Magnetic Immobilized β-Glucosidase

通过溶剂热法和改进的St?ber水解法制备了Fe_3O_4@SiO_2磁性纳米粒子,并用透射电镜对纳米粒子进行了表征.采用聚多巴胺辅助法将β-葡萄糖苷酶固定在磁性纳米粒子上.具体考察了加酶量、多巴胺质量浓度和固定化时间等因素对酶活和酶固定率的影响规律,获得了β-葡萄糖苷酶固定化的最适条件.结果表明,当加酶量为50,U/g、多巴胺质量浓度为1.6,mg/m L、固定化为24,h时,所制备的固定化β-葡萄糖苷酶活力为25.01,U/g,明显优于传统戊二醛交联固定的β-葡萄糖苷酶活力.

The Fe3O4@SiO2nanoparticles(NPs)were prepared through solvothermal and modified Stober methods,and the morphology of the nanoparticles was characterized by TEM.β-glucosidase was immobilized onmagnetic nanoparticles with the assistance of polydopamine.In this work,the effects of enzyme loadings,themass concentration of dopamine and the immobilization time on enzyme activity and immobilization yield weresystematically investigated.The results show that the immobilizedβ-glucosidase enzyme activity could reach25.01,U/g when enzyme loading was50,U/g,the mass concentration of dopamine was1.6,mg/mL and the immobilizationtime was24,h.It is superior to the enzyme activity ofβ-glucosidase immobilized by traditional glutaraldehydecrosslinking.