明日叶查尔酮对增强免疫力和抗运动性疲劳的效果

Immunoenhancing and Anti-Athletic Fatigue Effect of Ashitaba Chalcone

目的:研究明日叶查尔酮增强过度训练小鼠的免疫力及抗疲劳的能力。方法:建立雄性ICR小鼠8周跑台的过度训练模型,将模型小鼠分为4个免疫大组(共200只,每大组n=50),每个免疫大组又分为低、中、高3个剂量组(160、320、800 mg/kg)、过度训练组及阳性对照组(红景天西洋参胶囊200 mg/kg)(n=10),各剂量组以不同剂量的明日叶查尔酮灌胃并分别与过度训练组和阳性对照组进行比较,评价过度训练小鼠抗疲劳能力相关指标;并通过体液免疫、细胞免疫、单核巨噬细胞功能和自然杀伤细胞水平评价其免疫能力。结果:与过度训练组比较;明日查尔酮中、高剂量组的肌、肝糖原含量显著升高(P<0.05),血尿素氮含量、血清乳酸脱氢酶活力、血清肌酸激酶含量显著降低(P<0.05),并与阳性对照组没有显著差异;中、高剂量明日叶查尔酮能够提高自然杀伤细胞水平,促进小鼠抗体形成细胞增殖,促进脾淋巴细胞增殖转化。结论:明日叶查尔酮具有较好缓解运动性疲劳、增强免疫力的作用。

Objective:To investigate the effect of ashitaba chalcone on enhancing immune function and relieving fatigue in overtrained mice.Methods:A mouse model of overtraining was established using male ICR mice subjected to8-week treadmill training.The overtrained mice were divided into four groups with five small groups of10animals each:low-dose(160mg/kg),middle-dose(320mg/kg),high-dose(800mg/kg),model control and positive control(200mg/kg of Herba Rhodiolae-American ginseng capsule)groups.Ashaba chalcone was gavaged to the animals.The anti-fatigue effect of ashaba chalcone in overtrained mice was investigated by measuring related parameters,and we also evaluated the effect on immune function in terms of humoral immune parameters,cellular immune parameters,monocyte/macrophage functions and natural killer cell activity.Results:Compared with the overtraining group,the contents of muscle glycogen and hepatic glycogen significantly increased in the middle-and high-dose groups(P<0.05),and serum lactate dehydrogenase,creatine kinase and blood urea nitrogen decreased(P<0.05).Ashitaba chalcone at the middle and high doses could promote the proliferation of antibody-producing cells,the activity of natural killer cells,and the proliferation and transformation of mouse spleen lymphocytes.Conclusion:Ashitaba chalcone effectively ameliorates exercise-induced fatigue and enhances immune function.