FAS在结直肠癌组织与血清中的表达及与临床分期关系的探讨

The clinical discussion:expression of FAS in colorectal carcinoma tissues and relationship with clinical staging about expression in serum

目的通过观察FAS在结直肠癌组织与血清中的表达及其与临床分期关系,探讨FAS对评估结直肠癌预后的临床意义。方法选择经病理确诊的结直肠癌患者90例,对其癌组织与癌周组织,分别进行免疫组织化学检测和RT-PCR检测,比较FAS在癌组织与癌周组织中的不同表达;采用ELISA方法检测患者血清FAS和CEA水平,分析FAS与CEA的相关性;并选择同期体检的健康者90例,比较结直肠癌患者与健康者血清FAS水平的差异;根据以上90例结直肠癌患者术后病理诊断与临床检查进行临床分期,分析血清FAS水平与临床分期的相关性。结果免疫组织化学检测:FAS在癌组织中的表达远高于癌周组织中的表达,差异具有统计学意义(P<0.01);RT-PCR检测结果提示FAS在结直肠癌组织及癌周组织中的含量差异具有统计学意义(P<0.01);血清ELISA检测:结直肠癌患者血清FAS水平均显著高于健康者;结直肠癌患者血清FAS水平与其血清CEA水平具有相关性;结直肠癌患者血清FAS水平随着临床分期的增加而增加,两者之间呈正相关性(r=0.883,P<0.01);结论 FAS在结直肠癌患者癌组织及血清中均高表达,且与临床分期呈正相关性,提示FAS高表达预示着结直肠癌具有较高的侵袭性且患者预后不良,FAS对评判结直肠癌的生物学行为与预后具有一定的临床价值。

Objective Expression of FAS in colorectal carcinoma tissues and relationshi.Investigate the association between colorectal carcinoma tissues and serum levels of FAS in patients with colorectal cancer and clinicopathological characteristics of colorectal cancer.Explore the clinical significance of FAS to evaluate prognosis of colorectal cancer.Methods Select90patients with colorectal cancer that confirmed by pathology,select those patients'cancer tissue and peritumoral tissue,compare the expression of FAS in cancer tissues and peritumoral tissues by immunohistochemistry and RT-PCR;The levels of FAS and CEA in90patients with colorectal cancer were detected by ELISA in serum,analyze the correlation between FAS and CEA,choose90healthy person in the same period,compare the levels of FAS in serum in colorectal cancer patients and healthy person;90colorectal cancer patients'clinical staging were confirmed according to the pathological diagnosis and clinical examination,analyze the relationship with clinical staging about its expression in serum.Results Immunohistochemical staining,In the two groups(t=6.306,P<0.01),the difference was highly statistically significant.RT-PCR:FAS RT-PCR results the difference was statistically significant(t=5.927,P<0.01);RT-PCR results showed that FAS in colorectal cancer tissue and pericancerous tissue was significantly different;Serum ELISA test:the difference was statistically significant(t=16.553,P<0.01);It suggested that serum FAS level in patients with colorectal cancer were significantly higher than those in healthy person;Serum FAS level was positively correlated with serum CEA level(r=0.867,P<0.01).The serum level of FAS in patients with colorectal cancer is correlated with the level of serum CEA.The serum FAS level in patients with colorectal cancer increased with the clinical stage,there was a positive correlation between them(r=0.883,P<0.01).Conclusion FAS was highly expressed in colorectal cancer tissues and serum,and the expression of FAS was positively correlated with clinical stage,it suggested that FAS overexpression indicate colorectal cancer with high invasive and poor prognosis,FAS had the certain clinical value in evaluating the biological behavior and prognosis of colorectal cancer.