肝癌组织中MPDZ基因拷贝数和表达变化及其诊断预后价值分析

MPDZ copy number variations,expression and prognostic value in liver cancer

目的:探讨MPDZ基因在肝癌组织中的拷贝数和表达变化情况,并评价其与临床病理指标之间的关系以及对肝癌诊断和预后判断的价值。方法:利用TCGA数据库分析MPDZ基因在299例肝癌组织中的拷贝数改变情况及其与表达的关系;同时分析TCGA数据中MPDZ基因在282例肝癌组织和50例癌旁组织中的表达及其与临床病理指标和生存预后的关系。结果:MPDZ基因在肝癌组织中存在明显的拷贝数变异,且拷贝数变异的主要类型为拷贝数缺失(31.44%);同时MPDZ基因拷贝数缺失后MPDZ m RNA表达下调。MPDZ基因在肝癌组织中的表达显著低于癌旁组织,差异具有统计学意义(P<0.05)。Kaplan-Meier分析显示,MPDZ基因低表达患者的生存时间短于高表达患者,差异具有统计学意义(Log-rank=12.48,P<0.05)。受试者工作特征曲线(ROC)分析结果表明,MPDZ基因表达是一个灵敏度和特异度较好的肝癌诊断指标(AUC=0.86)。结论:MPDZ基因拷贝数缺失可能与肝癌的发生有关,MPDZ基因的表达对肝癌的诊断及生存预后判断具有参考价值。

OBJECTIVE:This study aimed to explore the copy number variations and expression of MPDZ and to evaluate their relationship with prognostic outcomes in liver cancer.METHODS:Copy number variations and expression of MPDZ were performed from TCGA database,and their relationships were analyzed.Expression of MPDZ in liver carcinoma(282cases)and adjacent normal tissues(50cases)were also performed,then the relationship between MPDZ expression and clinical pathological parameters,and its prognostic value were evaluated by statistical analysis.RESULTS:Copy number deletion(31.44%)was the most frequent copy number variations observed and it was associated with the expression of MPDZ.The expression was significantly lower than that in adjacent normal tissues(P<0.05).Kaplan-Meier survival curves showed that patients with low MPDZ expression had significantly shorter overall survival time than those with higher MPDZ expression(Log-rank=12.48,P<0.05).Receiver operating characteristic curve(ROC)showed that MPDZ gene expression was a sensitive and specific index of liver cancer diagnosis(AUC=0.86).CONCLUSION:MPDZ gene copy number deletion might be associated with the occurrence of liver cancer and the expression of MPDZ had some reference value for the diagnosis and prognosis of liver cancer.